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DIALAB PHOTOMETER DTN-410K USER MANUAL 

This instrument’s calculation routine is always a quadratic interpolation by using a set of ten 
different mathematical functions. The logarithm of concentration is an added feature. This 
enables graphic printouts of curve in semi-logarithmic format. 

 

Curve fitting is performed by Least Squares method applied to a quadratic function. 
 
The auto-calibration and incorporated control programs ensures maximum reliability of 
instrument’s output, and fast diagnostics and verifications. 
 
Measurement printout of operation methods, methods stored in memory, calibration curves 
and kinetics are obtained through the graphic printer, enhancing information displayed in the 
20x40 character liquid crystal display; backlight ensures comfortable viewing from any 
angle. 
 
A 25 key touchpad keyboard, co-related to displayed functions, provides user with simple 
and clear prompts. 
 
Serial RS232C interface allows bi-directional communication with PC IBM compatible 
computers and accessories. 
 
The equipment’s optical system consists of a highly collimated photometer, with stepper 
motor driven filter wheel, and high quality interferential filters. 
 
Factory pre-focused lamp reduces the need for tedious calibration after replacement. 
Calibration curves and factors are not affected by lamp change. 

 
 
1.2 Technical specifications 

 

Methods: 

 

 

Enzymes. Substrates. Electrolytes. Hormones. Drugs. 
 

Optical system: 

 

 

Principle: monochromator with interferential filters. 
Wavelength range: 340-1000 nm. 
Wavelength selection: automatic 9-positions filter wheel. 
Factory mounted filters: 340, 405, 450,505, 550, 620 and 750 nm. 
Bandwidth: 10 ± 2 nm. 
Photometric range: -0.5 to 4.000 Abs. 
Precision: +/- 0,002 Abs. 
Linearity: better than ± 1,5% from 0 to 1,500 Abs. 
Light source: halogen lamp 6 V 10 W. 
Zero: automatic. 
Reading: monochromatic and bichromatic. 

 
Cuvette system: 

Two –way cuvette system 
1. Metallic microflow cell: 18 ml  – 10 mm light path with temperature control by Peltier 
system. Temperature: variable, pre-adjusted 25ºC, 30ºC and 37ºC. Stability ± 0.1ºC. 

Содержание DTN-410 K

Страница 1: ...I 1 I DIALAB PHOTOMETER DTN 410K USER MANUAL PHOTOMETER DTN 410 K USER MANUAL ...

Страница 2: ...I 2 I DIALAB PHOTOMETER DTN 410K USER MANUAL ...

Страница 3: ...ibration 17 2 4 2 Flowcell verification procedure 18 3 Operating modes 21 3 1 Single sample photometer mode 21 3 2 Semi automatic analyzer mode 21 3 3 Methodologies 21 3 3 1 Method parameters 22 4 Photometer mode operation 25 4 2 Photometer mode main menu 25 4 3 Method preparation 1 set method 26 4 4 Measuring 2 Run Method 32 4 4 1 Methods with Standard 33 4 4 2 Methods with factor 35 4 4 3 Method...

Страница 4: ...ilter selection table 47 4 10 4 Delay ready Led 45 4 10 5 Automated cleaning ystem 46 4 11 Air Gap 49 5 Semi Automatic patient mode 50 5 1 Main menu overview 51 5 2 Methods settings and operation 51 5 2 1 Create Edit a method 51 5 2 2 Modifying method priority settings 52 5 2 3 Run method single method operation 52 5 2 4 Print Methods 52 5 3 Profiles definition 52 5 3 1 Adding new profiles 53 5 3 ...

Страница 5: ...ntenance 63 6 1 General care and daily maintenance 63 6 2 Flow through micro cell cleaning and care 63 6 2 2 Change and cleaning of sample filter 64 6 2 3 Flow cell disassembly and cleaning 65 6 3 Lamp replacement 67 6 4 Installing thermal paper roll 68 6 5 Pump tubing replacement 69 6 6 Identifying accessories and consumable goods 69 7 Troubleshooting 70 7 1 Non operating display 70 7 2 Message S...

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Страница 7: ... the instrument wear adequate lintless clothing Synthetic fabric aprons are recommended Filter reagents if they appear turbid after inspecting them against the light Follow the cell and filter cleaning instructions in the maintenance section when ending daily work Do not leave the flowcell empty overnight keep filled with distillate water 7 Clean flow cell and filter after daily work Please refer ...

Страница 8: ... used in the instrument Warning Before using read instructions in Manual Hazardous Voltage Ground connection Representative for CE CGA Strumenti Scientifici Via Luca Giordano 7b 50132 Florence FI Phone 39055571476 Fax 390555000889 E Mail Biological Risk ...

Страница 9: ... or samples of known concentration Antibody dosage and other substances that require a cutoff value can be performed provided there is a formula related to a negative and positive control The use of a calibration curve is recommended in all applications in which Lambert Beer s Law does not hold meaning a linear ratio between absorbance and concentration is not attainable Concentration values for t...

Страница 10: ...l RS232C interface allows bi directional communication with PC IBM compatible computers and accessories The equipment s optical system consists of a highly collimated photometer with stepper motor driven filter wheel and high quality interferential filters Factory pre focused lamp reduces the need for tedious calibration after replacement Calibration curves and factors are not affected by lamp cha...

Страница 11: ... system tests 30 non linear calibration curves Printer control Variable aspiration rate and sample recovery Automatic calibration and test mode Quality control Analysis modes Absorbance Concentration Endpoint with and without blank Sample and reagent blank Standard or factor Factor storage in all methods Non linear calibration mode Enzyme kinetics with and without sample blank Two point kinetics I...

Страница 12: ...I 12 I DIALAB PHOTOMETER DTN 410K USER MANUAL Dimensions 32 cm x 38 cm x 17 cm Weight 6 kg 1 3 Front view Graphic 1 1 shows the essential elements that compose the Analyzer 1 4 Rear view ...

Страница 13: ... and simplifcation of laboratory tasks Instrument stores up to 200 patients and up to 10 results per patient When external keyboard is not connected built in multifunction keyboard allows single sample operation as a standard spectrophotometer When pressing a non operative key a low pitch sound is emitted indicating the operational error The functions of the keys in the operational program are as ...

Страница 14: ...I 14 I DIALAB PHOTOMETER DTN 410K USER MANUAL ...

Страница 15: ...in good condition Please report non conformances to manufacturer or authorized dealer immediately 2 2 Main supply voltage The analyzer is a Class I instrument that requires 100 to 240 VAC 50 60 Hz This equipment requires a single phase line with protective ground connection Required fuse is type F fast for 3 amps 2 3 Cautions 1 Place the instrument in well ventilated environments free from dust an...

Страница 16: ...the lack of continuous use Place the tubes on the rotor once again 1 Connect the instrument to the main line with power chord Do not change the plug nor interrupt grounding connections 2 Install the equipment on a vibration free area leaving a space of at least 10 cm behind the instrument 3 Place a thermal paper roll in the printer see section 6 4 and connect external keyboard to instrument 4 Turn...

Страница 17: ...Instrument will ask for a graduate flask to perform volume calibration VOLUME CALIBRATION Insert tip on a graduate flask with at least 5ml of water ASPIR When ASPIR is pressed several consecutive aspiration cycles will be performed and once finished instrument will request to write the measured volume in uL and internal adjust of peristaltic turn to volume ratio will be calculated Volume calibrati...

Страница 18: ...be performed a From main menu press down arrow key on external keyboard several times and press enter key on Photometrics menu Measurement parameters can be modified according to the user s needs IMAGEN RETOCADA VOLUME 700 Press Next on built in keyboard and the following message shall appear IMAGEN RETOCADA VOLUME 700 Slide cell compartment cover open and select flow cell ...

Страница 19: ...eadings are stable within 0 005 Abs If readings are not stable aspirate solution 1 and wait for 2 or 3 minutes Then aspirate the same solution again aspirate soaking solution 2 several times 3 at least Finally aspirate water and verify stability of reading Pump Actuator In Wash method runs the pump In Run method aspirates blank standard and sample ...

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