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DIAGENODE
BIORUPTOR
®
PLUS
USER MANUAL
Diagenode Inc. North America
/ Phone: +1 862 209-4680 // Fax: +1 862 209-4681 // Mail: orders.na
@
diagenode.com
Introduction
Diagenode’s Bioruptor
®
Plus uses a gentle method of sonication to retain
the integrity of DNA and/or biological complexes, including chromatin,
protein-protein binding, protein-DNA complexes and other biochemical
and biological assay systems. The Bioruptor
®
Sonication System uses
a sonication bath to generate indirect sonication waves, which emanate
from an ultrasound element below the water tank. Because the system
is gentler than other sonicators, the Bioruptor
®
produces better and
more consistent results than with harsher sonication methods. Up to
12 closed tubes can be sonicated in parallel and the continuous rotation
of tubes allows even distribution of the energy for efficient sonication.
The Bioruptor
®
enables automation of sonication, guaranteeing higher
reproducibility of results.
The effect of ultrasound on biological samples.
The Bioruptor
®
sonication system uses ultrasound to create focused
mechanical stress to lyse cells or shear DNA or chromatin. Ultrasound
waves pass through the sample expanding and contracting the liquid.
During expansion, negative pressures pull the molecules away from
one another to form a cavity or bubble. This process is called cavitation.
The bubble continues to absorb energy until it can no longer sustain
itself and implodes. This produces intense focused shearing forces,
that disperse and break biomolecules. The fragmentation of DNA takes
place as a consequence of this mechanical stress or shear.
With the Bioruptor
®
, the entire volume of water present in the sonication bath is exposed to ultrasound, allowing all the
samples to be efficiently sonicated (Figure 1). For larger tubes (15 ml or 50 ml), the transfer of the ultrasound to the
tubes is facilitated by a metallic bar in contact with the sample. This metallic bar is not a probe (no corrosion problems)
but “reflects” the ultrasound originated from the sonication bath and improves the sample sonication efficiency by a
patented resonance system (Figure 2).
Noise level measurements & precautions
CEE noise data is not applicable to the Bioruptor’s ultrasound emitter as no tests have been conducted on similar
devices to date. See listed noise levels (as measured in the Accredited Acoustics Laboratory) and precautions for the
Bioruptor
®
:
L
eq
= noise level in dBA = 78.4 dB
L
max
= dB Peak = 87.6 dB
1. Exposure limit value
The exposure limit value is the maximum amount of noise an employee may be exposed to on any single day (8 hours).
Exposure beyond this limit presents a high risk to the user.
L
EXPOSURE
, 8h = 87 dB(A)
P
PEAK
= 200 Pa (140 dB(C) referring to 20 μPa)
Fig 1. Propagation in 0.5 ml tubes and 1.5 ml tubes
Ultrasound
Transducer
Ultrasound
Transducer
Fig 2. Propagation in 15 ml & 50 ml tubes
