NOTES
CytoSMART Lux2
Live Cell Imaging System
Instruction Manual
31
Страница 1: ...INSTRUCTION MANUAL Lux2 Your best friend in the lab...
Страница 2: ...ring the Cable 9 Status Indicator 9 Vessel Placement 9 Starting the Software 9 4 Setup an Experiment 10 Zoom Level 10 Focus 10 Registration of the Device 11 Account Setup 12 Start a Project 13 Start a...
Страница 3: ...25 Removing Dust Particles from Optical Window 25 Troubleshooting 25 Please connect the device 26 Please connect the charger 26 Please connect to the internet 26 Server offline online functionality un...
Страница 4: ...mmediately Do not attempt to repair or remove the outer case of the device If you open this instrument you put yourself at risk of bodily harm due to electrical shock in addition to damaging the devic...
Страница 5: ...n several minutes and requires minimal training Hardware and software work together like a charm The system consists of a monitoring device which operates inside a CO2 incubator The device is linked t...
Страница 6: ...is ideal for monitoring migration assays or hypoxic cell cultures The system can also be used for cell growth curves and the screening of effects from culture media and growth factors System Overview...
Страница 7: ...5 License key Remember if you ever have any questions regarding your purchase please visit https www cytosmart com support or contact our support staff via support cytosmart com We are happy to help...
Страница 8: ...the device Cable Placement Check if the incubator has a cable port in the back If so guide the USB cable from the CytoSMART Lux2 through it fig 3a If no port is present place the cable between the do...
Страница 9: ...he device is powered down as it is in between imaging or not properly connected Vessel Placement It does not matter which culture vessel you use The CytoSMART Lux2 is compatible with a variety of cult...
Страница 10: ...like Select the correct zoom level for the experiment Just select Menu 1 on the bottom left corner and select Zoom in or Zoom out 2 for a 200x or 100x magnification respectively Fig 5 Focus The right...
Страница 11: ...select New registration 6 To register the CytoSMART Lux2 enter a name 7 for the device e g company name or research group Other CytoSMARTTM products can be added to this name Next create a personal ac...
Страница 12: ...session of a cell culture experiment Select Start Experiment 4 in the live screen fig 5 to start a new experiment simply login A menu will appear Fig 9 asking to provide login details 13 Use the detai...
Страница 13: ...al users will receive an invitation email to join the project You need to provide a project name to continue 18 Next an optional project description and visual representation Project Icon can be added...
Страница 14: ...Snapshot interval in minutes 25 A drop down menu will appear with different intervals to choose from Important Since biological cells can be susceptible to light it is advised not to use a higher reco...
Страница 15: ...oud If you want to access the cloud the following are required PC tablet or another device with a web browser Active internet connection CytoSMART Cloud login details A two year subscription to the Cy...
Страница 16: ...t use the Search Projects bar 1 to quickly search for a project To create a new project select New Project 2 This is similar to when beginning a new project as described in 4 It is not possible to sta...
Страница 17: ...within a project Select a specific experiment by clicking on its Experiment Icon Experiment Title or the Arrow Icon 8 This will bring up the experiment page of that specific experiment To edit details...
Страница 18: ...of the panes and buttons in this Experiment Page Overview 11 Time lapse movie of the cell culture 12 Download buttons to download movies mp4 images jpeg and cell coverage and temperature values csv 13...
Страница 19: ...Tracking button allows to display the cell coverage in the time lapse movie Adjust the playback speed of time lapse movie with the Turtle Cheetah slider Experiment Summary Pane The summary pane fig 1...
Страница 20: ...if Cell coverage reaches a specified percentage Temperature crosses a specified limit To set a notification select the button The notification pane will unfold allowing you to specify the Type and Va...
Страница 21: ...overage in percentage of surface area is plotted against the duration of the experiment fig 23 Temperature Graph In this graph the temperature C is plotted against the duration of the experiment fig 2...
Страница 22: ...perform analysis or set notification thresholds of the images during an offline experiment Zoom and Focus Before you start an offline experiment adjust the zoom level and focus setting for the experim...
Страница 23: ...intervals to choose from Important Since biological cells can be susceptible to light it is advised not to use a higher recording frequency than needed When all details are set select the Start butto...
Страница 24: ...e screen To minimize the CytoSMARTTM software select Menu and then Minimize 7 This will open a folder containing all the images jpg of the offline experiments Fig 29 You can review the image files fro...
Страница 25: ...ty Important Do not use acetone to clean the CytoSMART Lux2 Also do not autoclave the CytoSMART Lux2 this can result in damage to the electronics The CytoSMART Lux2 does not need regular maintenance D...
Страница 26: ...start the tablet again When using a USB hub be aware that some older versions e g non powered USB hubs might not provide enough power for the Lux2 to operate properly Please connect to the internet W...
Страница 27: ...prolonged exposure to light No Project Email Received A confirmation email should arrive a few minutes after starting an experiment If no email was received please check to see if the SPAM filter blo...
Страница 28: ...nm 5 MP CMOS 10 1 inch tablet No optical filters 133 x 90 x 100 mm LxWxH or 5 2 x 3 5 x 3 9 LxWxH 0 5 kg or 1 1 lbs Unlimited storage in the CytoSMART Cloud with the subscription 5 40 C 20 95 humidit...
Страница 29: ...one by anyone other than CytoSMARTTM or an authorized agent 6 Damage caused by substituting alternative parts 7 Use of fittings or spare parts supplied by anyone other than CytoSMARTTM 8 Damage caused...
Страница 30: ...om CytoSMARTTM for the duration of 1 year 2 years or 5 years CytoSMARTTM Lux2 Instruction Manual 30 Cat No HCA 1003 HCB 1003 HCC 1003 Description CytoSMARTTM Care Lux2 option 1 CytoSMARTTM Care Lux2 o...
Страница 31: ...NOTES CytoSMART Lux2 Live Cell Imaging System Instruction Manual 31...
Страница 32: ...e marks of CytoSMART Technologies or its affiliates The information contained herein is believed to be correct and corresponds to the latest state of scientific and technical knowledge However no warr...
