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2. 3. バンドの確認    Checking sample bands

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電気泳動槽、ゲルキャスティングトレイには
紫外線透過性樹脂を使用しているので、予め
ゲルに染色液を加えている場合、ゲルを電気
泳動槽から取り出すこと無くトランスイルミ
ネーターでバンドを確認できます。

  紫外線から眼、皮膚を保護するためのバイザーやゴー
グルなどを使用してください。

ゲルに染色液を加えていない場合は、ゲル
キャスティングトレイからゲルを取り出し、
必要時間染色してください。

  

DNA の染色にエチジウムブロマイドを使用する際

は必ずグローブを着用し、皮膚に触れないようにしてく
ださい。

染色後、トランスイルミネーターで観察してくだ
さい。

When staining is completed, observe the result 
using transilluminator.

Due to UV (Ultraviolet) light transparency of 
Gel electrophoresis tank and Gel casting tray, 
the result can be observed using 
transilluminator without taking out the gel 
from the tank, if staining dye has been added 
into the agarose gel when preparing it. 
  

     

Always wear a visor or approved goggles to help 

vision and to protect your eyes and skin from UV light.

If staining dye has not been added into the
agarose gel when preparing it, take out the
gel from Gel casting tray and stain it for
necessary time length.

      

When using ethidium bromide for DNA staining, 

always wear gloves to avoid skin contact.

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i-MyRunⅡ核酸電気泳動システム取扱説明書̲B5.indd   12

i-MyRunⅡ核酸電気泳動システム取扱説明書̲B5.indd   12

2014/10/09   14:50:01

2014/10/09   14:50:01

Содержание i-MyRun II

Страница 1: ...i MyRun II...

Страница 2: ......

Страница 3: ...N SIGN WARNING SIGN Electricity Indicates instructions to follow Important user information Indicates a potentially hazardous situation which if not avoided will result in death or serious injury Indi...

Страница 4: ...l shock Do not use this product if outer damage cuased by strong shocks such as from being dropped as a re or electrical shock could result Do not operate this product nor unplug Power cable from the...

Страница 5: ...bu er in it separate the Power supply rst before moving unless hold the Power supply and the tank rmly with both hands as electrode connectors will be stressed equipment damage or dropping of product...

Страница 6: ...eave bu er in Gel electrophoresis tank for prolonged period as degradation of electrode could result Gel electrophoresis tank and Gel casting tray both have UV Ultraviolet light transparency though pr...

Страница 7: ...Features Power supply Gel electrophoresis tank Gel electrophoresis tank cover Gel casting tray Combs Gel casting stand Power cable 1 1 Standard components 1 1 each 1 1 each 1 1 each 1 1 each 6 6 each...

Страница 8: ...rom Gel electrophoresis tank Power supply will detach from the Gel electrophoresis tank by rotating towards it How to attach Power supply and Gel electrophoresis tank When attaching Power supply slide...

Страница 9: ...ns 0 99 0 Timer settings from 0 99 minutes Gel electrophoresis will end when timer reaches 0 zero When timer is set to 0 zero will be displayed for continuous run mode 3 Voltage setting button 50 75 1...

Страница 10: ...or 0 5 TAE bu er is recommended For preparation of 4 mm thickness agarose gel amount of 70 80 mL gel will be required For 4 mm thickness agarose gel 9 L 26 well comb or 18 L 13 well comb of sample ca...

Страница 11: ...tore agarose gel you have prepared be sure to cover the gel with bu er after gently drawing out Comb s Wrap the gel in plastic wrap and store it under low temperature Remove Gel casting tray with agar...

Страница 12: ...electrophoresis Please double check that RUN button lights up and air bubbles forming from platinum wires at both ends of the tank Performing gel electrophoresis for prolonged time under condition of...

Страница 13: ...t taking out the gel from the tank if staining dye has been added into the agarose gel when preparing it Always wear a visor or approved goggles to help vision and to protect your eyes and skin from U...

Страница 14: ...W 65mm H 240 mm L Gel electrophoresis tank UV light transparent resin Gel casting tray UV light transparent resin Gel electrophoresis tank cover UV light un transparent resin Not enough current in th...

Страница 15: ...d strains tilts Make sure that agarose gel does not contain any air bubbles nor undissolved gel blocks Make sure that agarose gel is not too thin For preparation of 4 mm thickness agarose gel amount o...

Страница 16: ...If observing the result through gel electrophoresis tank or tray try observing only the gel If the gel after gel electrophoresis has been left out for prolonged period nucleic acid bands may di use an...

Страница 17: ...memo 16...

Страница 18: ...i MyRun II e mail FAX 1 i MyRun II IMR2 001 FAX TEL e mail 17...

Страница 19: ...1 2 3 4 18...

Страница 20: ...0016 2 2 20 TEL 03 5632 9610 FAX 03 5632 9619 TEL 03 5632 9620 URL http www cosmobio co jp TOYO EKIMAE BLDG 2 20 TOYO 2 CHOME KOTO KU TOKYO 135 0016 JAPAN TEL 81 3 5632 9617 FAX 81 3 5632 9618 email...

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