Cleaver Scientific MSMIDI96 Скачать руководство пользователя страница 14 | Manualshive

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Cleaver Scientific MSMIDI96 Скачать руководство пользователя страница 14

15/11/2018

Page 12

10X TBE stock (1L)

dissolve in 750 ml distilled water:



108 g tris base (FW = 121)



55 g boric acid (FW = 61.8)



40 ml 0.5 M EDTA (pH 8.0)

Fill to 1 litre final volume with distilled water

Loading Dye

10x sample buffer stock consists of 50% glycerol, 0.25% bromophenol blue,
and 0.25% xylene cyanole FF in 1x TAE buffer. Only 1–10 ml of the 10x loading
dye should be prepared.

Ethidium Bromide Solution

Add 10 mg of Ethidium Bromide to 1 ml distilled water.

References

1. Sambrook, Fritsch, and Maniatis,

Molecular Cloning A Laboratory

Manual,

Second Edition, Cold Spring Harbor Laboratory Press, 1989.

2.

Current Protocols in Molecular Biology,

Greene Publishing Associates

and Wiley-Interscience, 1989.

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Содержание MSMIDI96

Страница 1: ...multiSUB Midi96 Horizontal System ...

Страница 2: ......

Страница 3: ... 1 5 2M MSMIDI96ST MSMIDI96ST1 5 MSMIDI96ST 2M MSMIDI96ST 1 5 2M Record the following for your records Model _____________________ Catalogue No _____________________ Date of Delivery _____________________ Warranty Period _____________________ Serial No _____________________ Invoice No _____________________ Purchase Order No _____________________ ...

Страница 4: ...6 Setting up the Horizontal Gel Tanks 6 Fitting Electrodes 6 Fitting Loading Guides 6 Gel Preparation 7 Gel Pouring 8 Casting Dams 8 Flexicaster 9 Tape 10 Running the Gel 10 Gel Staining and Viewing 11 Solutions 11 References 12 Troubleshooting 13 Care and Maintenance 14 Cleaning Horizontal Units 14 RNAse Decontamination 14 Ordering information 15 Combs 15 Related Products 16 Warranty 17 ...

Страница 5: ...the complete manual thoroughly The unit must never be used without the safety lid correctly in position The unit should not be used if there is any sign of damage to the external tank or lid These units comply with the following European directives 2006 95 CE Low Voltage Directive and 2014 30 UE official Title 2004 108 EC EMC Electromagnetic Compatibility By virtue of the following harmonised stan...

Страница 6: ...x L MS10 UVDAM Pack of 2 MSMIDI96ST 8 1 5 CB MS10 LG Strips MS10 WP Platform CSL CAB MSMIDI96S T 2M MS10 UV96ST 10 x 24cm W x L MS10 UVDAM Pack of 2 MSMIDI96ST 8 1 2M CB MS10 LG Strips MS10 WP Platform CSL CAB MSMIDI96S T 1 5 2M MS10 UV96ST 10 x 24cm W x L MS10 UVDAM Pack of 2 MSMIDI96ST 8 1 5 2M CB MS10 LG Strips MS10 WP Platform CSL CAB Packing List Checked by ________________________ Date _____...

Страница 7: ...ent gel tray UV transparent above 300nm Moulded acrylic 5 Comb Slots Allow multiple combs per tray 6 Casting dams Natural rubber 7 Colour coded electrodes Moulded acrylic 99 99 platinum wire gold plated 4mm plugs 8 Gel platform Notched to fix gel position 9 Safety lid thumb locators For safe removal of lid 10 Moulded buffer tank High Impact moulded acrylic construction for chemical compatibility a...

Страница 8: ...s Fitting Electrodes Note the position of the lid on the unit This shows the correct polarity and the correct orientation of the cables black is negative and red positive Remove the lid from the unit Note if the lid is not removed fitting the cables may result in un tightening of the gold plug and damage to the electrode Screw the cables into the tapped holes as fully as possible so that there is ...

Страница 9: ...arose powder to a conical flask 2 Add the appropriate amount of 1x TAE or TBE solution from the table above To prevent evaporation during the dissolving steps below the conical flask should be covered with parafilm 3 Dissolve the agarose powder by heating the agarose either on a magnetic hot plate with stirring bar or in a microwave oven If using the microwave method the microwave should be set at...

Страница 10: ... no possibility of gel leakage 2 Place the comb s in the grooves Each tray has more than one comb grove so that multiple combs can be used Using multiple combs increases sample number available per gel but decreases run length and care must be taken to ensure that samples from the first wells do not migrate into the lanes of the second comb wells 3 Pour in the agarose carefully so as not to genera...

Страница 11: ...Flexicaster so that the silicone mat is pushed against the other end of the tray 4 Turn the cam so that the silicone mat tightly seals against the side of the tray Pour in the agarose carefully so as not to generate bubbles Any bubbles that do occur can be smoothed to the edge of the gel and dispersed using a pipette tip 5 Allow the agarose to set ensuring that the gel remains undisturbed 6 Carefu...

Страница 12: ...tip 4 Allow the agarose to set ensuring that the gel remains undisturbed 5 Carefully remove the gel casting gates and comb and transfer the gel including tray to the main tank Running the Gel 1 Mix the sample to be loaded with sample buffer see solutions for common sample buffers Usually 3ul of sample buffer is adequate but less may be used with sample volumes of less than 10ul 2 Fill the unit wit...

Страница 13: ...ersed 3 Rinse the gel twice for a couple of seconds with distilled water 4 Transfer the gel to a UV Transilluminator The samples will often appear as brighter clearer bands when photographed or viewed using a gel documentation system However if the gel bands are too faint then the staining procedure should be adjusted so that there is less de staining If there is too much background then the stain...

Страница 14: ...glycerol 0 25 bromophenol blue and 0 25 xylene cyanole FF in 1x TAE buffer Only 1 10 ml of the 10x loading dye should be prepared Ethidium Bromide Solution Add 10 mg of Ethidium Bromide to 1 ml distilled water References 1 Sambrook Fritsch and Maniatis Molecular Cloning A Laboratory Manual Second Edition Cold Spring Harbor Laboratory Press 1989 2 Current Protocols in Molecular Biology Greene Publi...

Страница 15: ...e running surface Curved line or distortion of bands Bubbles in sample wells Remove bubbles prior to electrophoresis Curved bands smiles Sample overload Reduce load Differential relative mobilities Sample spilled out of wells Unit not levelled Samples should have proper density Apply carefully Level unit Use a steady work bench Gels crack Too high voltage gradient especially with low melting tempe...

Страница 16: ...rgents include dishwashing liquid Hexane and Aliphatic hydrocarbons The units should not be left to in detergents for more than 30 minutes The units should never come into contact with the following cleaning agents these will cause irreversible and accumulative damage Acetone Phenol Chloroform Carbon tetrachloride Methanol Ethanol Isopropyl alcohol Alkalis RNAse Decontamination This can be perform...

Страница 17: ...omb 8 sample MC 1mm thick two marker lanes MSMIDI96 8 1 5 2M MSMIDI 96 Comb 8 sample MC 1 5mm thick two marker lanes MSMIDI96ST 8 1 CB MSMIDI 96 STRETCH Comb 8 sample MC 1 Marker 1mm thick COMB BLOCK MSMIDI96ST 8 1 5 CB MSMIDI 96 STRETCH Comb 8 sample MC 1 Marker 1 5mm thick COMB BLOCK MSMIDI96ST 8 1 2M CB MSMIDI 96 STRETCH Comb 8 sample MC 2 Marker 1mm thick COMB BLOCK MSMIDI96ST 8 1 5 2M CB MSMI...

Страница 18: ...0 5g tablets CSL RUNSAFE CSL RUNSAFE Package 1 ml vial CSL TBEP Powdered Tris Borate EDTA Running Buffer 10 sachets 1litre pk TBE10X1L Cleaver Buffer Tris Borate EDTA Running Buffer 10 x 1L TBE10X5 Cleaver Buffer Tris Borate EDTA Running Buffer 10 x 5L TAE50X1L Cleaver Buffer Tris Borate EDTA Running Buffer 50 x 1L TAE50X5L Cleaver Buffer Tris Borate EDTA Running Buffer 50 x 5L CSL MDNA 100BH 100b...

Страница 19: ...en performed by anyone other than CSL or an appointed distributor or representative are no longer under warranty from the time the unit was modified Units which have accessories or repaired parts not supplied by CSL or its associated distributors have invalidated warranty CSL cannot repair or replace free of charge units where improper solutions or chemicals have been used For a list of these plea...

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