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INSTRUCTION MANUAL

TLC-MS INTERFACE 2

Содержание TLC-MS INTERFACE 2

Страница 1: ...INSTRUCTION MANUAL TLC MS INTERFACE 2...

Страница 2: ...Maintenance and Service 10 4 1 Cleaning 10 4 2 Decontamination 10 4 3 User maintenance 11 4 4 Maintenance Data Sheet 11 4 5 Replacing of elution head 12 5 Technical data 12 6 Application hints 13 6 1...

Страница 3: ...ive detergents Only authorized personnel may open the instrument Service and repair is only to be performed by trained specialists Use spare parts and consumables supplied by CAMAG only The warranty i...

Страница 4: ...are kept in the equipment for a long time electrolyte leakage may occur and cause damage to the instrument Laser class 1 inside Do not look directly into the laser beam 1 2 Parts supplied Part no Desc...

Страница 5: ...from HPLC pump to TLC MS interface 2 and from there to the MS Bench space Width 27 5 cm Depth 45 cm Height 30 cm Operating temperature The temperature should be within a range of 15 to 35 degrees cent...

Страница 6: ...tion Manual Feb 17 5 2 4 Connection of the valve Bypass position Elution position 1 2 3 4 5 6 From LC pump Compressed air at cleaning To inline filter and LCMS Color sleeve Elution head Internal filte...

Страница 7: ...this manual 3 2 Instrument Fig 2 Control elements 1 Laser cross hair on off 2 Piston up down 3 Cleaning on off 4 Screw for fixing the security cover 5 Piston with elution head 6 Positioning table 7 Le...

Страница 8: ...ght of about the thickness of the plate layer When the elution head is pressed onto a foil or glass plate the cutting edge seal cuts into the adsorption layer and creates a leak free seal In bypass po...

Страница 9: ...he cleaning button at least 3 times for 3 seconds The capillary from the plate to the valve and internal frit are cleaned with the help of compressed air At the same time the cleaning sledge moves und...

Страница 10: ...r and ruler on the instrument table accordingly Toggle the Piston up down switch to lower the elution head onto the plate foil Switching the valve to front Elution position will start the elution An e...

Страница 11: ...on a regular basis in an appropriate way assures problem free functionality over a long period A good way to perform a cleaning would be to follow the decontamination instruction outlined below 4 2 De...

Страница 12: ...trument as well as the proposal for IQ OQ interval if applicable In addition it identifies parts subject to wear with the respective replacement cycle and CAMAG order number Maintenance interval Maint...

Страница 13: ...blue top cover Mount the security cover Make an fake extraction to check if the elution head is properly positioned and no leaks are visible 5 Technical data General data Plate types TLC HPTLC glass...

Страница 14: ...alve and port 1 directly to the MS it is recommended to bypass the UV detector cell because it is sensitive to back pressure from the TLC MS Interface 2 It is highly recommended to use an inlet filter...

Страница 15: ...et a clean mass spectrum Fig 6 Flow injection analysis FIA of 1 L of 0 113 mg mL diclofenac in methanol upper curve total ion current TIC lower curve single ion monitoring SIM of the masses 296 00 297...

Страница 16: ...different samples on the same plate a short band of 4 mm may be adequate For fewer samples which are to be analyzed more than once a band length of 10 to 20 mm is preferred Alternatively samples can...

Страница 17: ...the smaller tubing in receiving ports normally designed for the larger 1 16 OD tubing Generally the high pressure receiving ports in most of the equipment on the market have an internal geometry supp...

Страница 18: ...ry tubing into a receiving port is the method of preference unless the receiving port is specifically designed for use with the size of tubing you are using 6 3 Dead volumes One of the concerns you wi...

Страница 19: ...d ferrules require a wrench to tighten them even after repeated use Finger tight fittings also require a good turn however don t use a wrench unless instructed to do so or you may damage the fitting N...

Страница 20: ...54nm mark zones with a soft pencil Fig 11 Different volumes of 0 113 mg mL diclofenac in methanol 8 mm bands MS measurement Start the HPLC pump The eluent used in the presented example was 95 methanol...

Страница 21: ...the process This procedure allows you to register a series of samples into one MS file Fig 12 Elution of three consecutive sample zones of diclofenac 2 4 and 10 L per 8 mm band Upper curve TIC lower...

Страница 22: ...e 60 mm drying time 5 minutes Documentation CAMAG Visualizer or DigiStore 2 Documentation System at 254nm Fig 14 Separation of Paracetamol Caffeine Acetylsalicylic Acid mixture from left to right 25 g...

Страница 23: ...ted manually CAMAG TLC MS Interface 2 The selected sample zone is positioned below the elution head either by means of the laser crosshairs or by means of the x y scale The elution head is lowered ont...

Страница 24: ...the TIC total ion current is registered Known substances can be measured with a much higher sensitivity if only the ion of interest e g the ion with a m z of 195 1 Da in the case of caffeine In this...

Страница 25: ...tion of the TIC total ion cur rent baseline shows relative high noise Lower part MS of caffeine with APCI ionization M H ion 195 1 Da is still clearly visible background noise from the plate reaches a...

Страница 26: ...7 34 34 Fax 41 61 461 07 02 E Mail info camag com CAMAG Germany Bismarckstra e 27 29 D 12169 Berlin Telephone 49 30 516 55 50 Fax 49 30 795 70 73 E Mail infoberlin camag com CAMAG Scientific Inc USA 5...

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