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2.1) OptoSplit panels

B: Left side panel

A: Top Panel

BY

PASS

 

SPLIT

 

OPTOSPLIT

cairn-research.co.uk

Bypass

Bypass / split lever

XY controls for Bypass image

Split control

Rectangular aperture 

adjusters

SPLIT

REFLECTED PATH

V

H

Reflected H & V controls

Split control

Door cover

Support leg

Содержание OPTOSPLIT II BYPASS

Страница 1: ...uneven illumination which is often not introduced by the image splitter Once both your transmitted and fluorescent light source have been correctly aligned and focussed the OptoSplit can be mounted o...

Страница 2: ...OptoSplit using the two screws provided 2 Screw the support legs to the end of the bar 3 The height can be adjusted on each support leg we recommend a spirit level is used for final adjustment 2 Key...

Страница 3: ...arch co uk 2 1 OptoSplit panels B Left side panel A Top Panel BYPASS SPLIT OPTOSPLIT cairn research co uk Bypass Bypass split lever XY controls for Bypass image Split control Rectangular aperture adju...

Страница 4: ...d to ensure the Bypass image is projected onto the centre of the camera sensor It s best practise to ensure this is carried out prior to performing dual channel imaging but this adjustment can be chec...

Страница 5: ...door cover 3 Adjust the two rectangular aperture adjusters one horizontal the other vertical to create a single rectangular region of interest visible on your camera 4 Ensure the image is square with...

Страница 6: ...imaging software is useful as a reference tool to ensure the image is centred and square 4 Obtaining a split image The predominant operating mode of two identical images projected onto each half of y...

Страница 7: ...nd horizontally using the provided 1 5mm allen key on the H V controls on the magnetic door cover Once you are aware which channel is which turn your attention to the transmitted image and position th...

Страница 8: ...l ensure maximal field of view is projected onto each half of your camera sensor Vignetting may be present once split mode has been optimised This is characterised by one image appearing darker on one...

Страница 9: ...end the Chroma Ultra Flat UF 2 2mm thick dichroic mirror range The Cairn Filter Cube accepts dichroic mirrors of the following size 26mm x 38mm x 2mm Other dichroic mirrors will fit but may not be opt...

Страница 10: ...3 Remove the blank cover for the auxiliary drop in position reflected path Note the blank hold ers will have no angled thread indictor and act as a protector for this pocket 4 Insert the rotating pola...

Страница 11: ...ugins folder in your root MicroManager folder on the C drive A MicroManager plugin for use with version 2 0 is also currently in development Keep an eye on our website for more details Please contact...

Страница 12: ...ange your microscope cube to a position suitable for Cy5 imaging 2 The mCherry emission filter will need to be removed as it will impair the Cy5 channel For ease of removal we recommend mounting the m...

Страница 13: ...f required refer to section 3 2 5 Open the rectangular aperture To return to Split conditions 6 Slide the Bypass Split lever to Split 7 Replace the Cairn filter cube 8 Return your microscope cube to a...

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