email: [email protected] [email protected]
+44(0)1795 590140 www.cairn-research.co.uk
7.3) Obtaining a full field image of a shorter emission wavelength than your split conditions
OR imaging a variety of dyes utilising the microscope cubes.
e.g. A full field DAPI image when splitting GFP / mCherry
1.
Remove the Cairn filter cube, gaining access via the magnetic
door cover
.
2.
Change your microscope cube to a position suitable for DAPI imaging.
3.
Slide the
Bypass / Split lever
to ‘Bypass’
4.
Centre the Bypass image (if required) – refer to section 3.2
5.
Open the
rectangular aperture
.
To return to ‘Split’ conditions:
6.
Slide the
Bypass / Split lever
to ‘Split’
7.
Replace the Cairn filter cube
8.
Return your microscope cube to a position suitable for use with the OptoSplit
9.
Close the
rectangular aperture
to ensure only half the camera chip is illuminated.
10.
Although designed to generate minimal displacement when replacing the Cairn filter cube,
final adjustment of split conditions may be necessary – please refer to section 4.2
Please refer to the supplementary Troubleshooting Guide for additional information not de-
scribed in this manual.
Do not hesitate to contact us if you have any questions [email protected]
445mm
60mm
130mm
135mm
