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Inversed Biological

Microscope

BS-2090

Instruction Manual

This manual is written for Inversed Biological Microscope BS-2090. For safety and for
exerting the best performance, making you familiar with the instrument entirely, it is
strongly recommended that you read this manual carefully before using the microscope. For
the further reference, please place this manual in a position where nearby the worktable and
fetched easily.

BioTools Inc.

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Содержание BS-2090

Страница 1: ...S 2090 For safety and for exerting the best performance making you familiar with the instrument entirely it is strongly recommended that you read this manual carefully before using the microscope For the further reference please place this manual in a position where nearby the worktable and fetched easily BioTools Inc ...

Страница 2: ...he dust cap to prevent the dust and the splashed 1 Do not keep the instrument in a direct sunlight high temperature or humidity dusty and easy shaking environment Make sure the stage is plane horizontal and stable enough 2 When moving the microscope please hold up the instrument with one hand on the lower side of the eyepiece tube and the other hand on the illumination bracket figure1 3 If the bac...

Страница 3: ... with the dust casing And make sure the lamp cools down enough before you do so Ⅲ Safety Symbol g n i n a e M l o b m y S The surface is very hot not touch by your hands Before using please read the instruction carefully improper operation will result in bodily injure or instruction malfunction The main switch on The main switch off ...

Страница 4: ...hase contrast slider Objective Lamp house Microscope base Stage fixed Objective turret Nosepiece fixed Stage lengthen splint Glass stage Quintuple Trinocular viewing tube fixed 4X 10X PH 20X PH 40X Ocular eyepiece Condenser Stage inserted plate Illumination bracket ...

Страница 5: ... installation steps Before installing be sure every components is clean do not score any parts or glass surface Keep well with the supplied hexagon wrench When changing the components you will need it again Figure 2 Lamp house Microscope base Stage lengthen splint Mechanical ruler Power cord Objectiv Glass stage Ocular eyepiece Filter holder Condenser illumination unit Color filter ...

Страница 6: ...after When using or soon after it is turned off the lamp the lamp house and nearby parts will be very hot and will cause serious burns Please turn the main switch on O off pull up power plug and make sure the bulb the lamp house and periphery are all cool Then you can do your replacing Please insert the lamp gently or it will be damaged by excessive extrusion Do not touch the Halogen bulb with you...

Страница 7: ...holder facing forwards and keep the screw of condenser illumination unit and the hole of the holder in line then screw down the bolt in the hole with the supplied hexagon spanner 3 Insert aviatic BNC connector plugs into aviatic BNC connector jack 2 2 3 Installing the lamp house figure 5 Keep the BNC connector plugs and the lamp house pin in line and keep the bolt and the condenser jack in line to...

Страница 8: ...objectives according the magnification sequence of low to high Mount objective like this way will make the change of magnification to be very easy in using It also can install the objective through the stage opening Clean the objective regularly the objective used in the inversed microscope is very sensitivity about dust Do cover all the unused holes with turret dust caps to prevent the dust and c...

Страница 9: ...you can t install the mechanical ruler together Generally the mechanical ruler will be installed in the right side for comfortable adjustment 1 Installing the stage lengthen splint First Screw the fixed bolt on to the splint then mount it on to the stage from right or left below screwing down it until it stay hard 2 Installing the mechanical ruler Please install the ruler like the way of the stage...

Страница 10: ...e 2 Insert the eyepiece into tube until they are against 2 2 8 Installing the color filters fig 11 Be sure the color filter cools down completely before you change them Take down the filter holder then install the color filters you need Mount the color filter downwards like shown keep it horizontal through the end not allow inclined If the color filter is inclined or not get to the end it will dro...

Страница 11: ...upply receptacle Make sure the connection is well 4 Insert aviatic BNC connector plugs into aviatic BNC connector jack Do use the supplied power cord all the time If lost or damaged select the same standard cord please Connect the power cord correctly to ensure the instrument is earthing 2 2 10 Replacing the fuse fig 12and 13 Do remember to turn the main switch on the state of O off before replaci...

Страница 12: ...stment BS 2090 Figure 15 Diopter ring Light path selector lever Light switch Tension adjustment collar Main switch Coarse focus knob Fine focus knob Phase contrast slider Color filter holder Aperture diaphragm adjust lever Phase center bolt ...

Страница 13: ...ng the service life 4 1 3 Adjusting the Tension Adjustment Collar fig 18 The tight tension of the coarse focus knob had already adjusted before leaving factory How to adjust the tight tension Turning the tension adjustment collar while revolving at the direction which shown by the arrowhead on the figure the tight tension of the coarse focus knob is increasing and if at the contra direction the ti...

Страница 14: ... the bottom thickness is 1 2mm and the same thickness is also required by the object slide when it is laid the specimen using the Φ35mm culture dish You can lay aΦ35mm culture dish on the stage directly by using the standard center board of the stage using the mechanical ruler 1 When using the 96bit or 24bit micro titration board please fasten it tightly by the stage clips 2 When fastening other m...

Страница 15: ... adjustment ring The number which the reticle on the eyepiece holder pointed is your eye s diopter graduation 4 3 2 Adjusting the interpupillar distance fig 22 When observing with two eyes hold on the left and right prism holder turn around the axis adjusting the interpupillar distance until the left and right fields of view coincide completely The reticle on the interpupillar distance indicator p...

Страница 16: ...deo or photography pulling out the lever until it reached the clicked position Light path selector lever Brightness proportion application Pushing in the lever until it reached the limit position 100 used for binocular observation Binocular observation Pulling out the lever until it reached the limit position 20 used for binocular observation and 80 used for video or photography Binocular observat...

Страница 17: ...using the LBD color filter which can compensate more neutral colors You could pile up a group of color filters to the filter holder if you ensure they are level and the whole thickness is less than 11mm Color filter meaning IF550 Single contrast color filter green used for the phase contrast microscopy LBD Color temperature transit color filter blue used for bright field observation and microphoto...

Страница 18: ...colored yo Best fig 25 e aperture di erical apertur er image reso u could remo into the view changed by d diaphragm specimen you l aperture wh ou could turn tscope In iaphragm con re of the obj olution and co ove the eyepi wing tube yo dialing the ap is the edg u need to set hich marked in the aperture nternation ntrol the num ective and th ontrast and t iece if neces ou might see a perture adjust...

Страница 19: ... contrast objective please see 2 2 4 You ought to mount it on the turret 5 1 2 Phase contrast slider fig 27 phase centering adjustable slider The light ring was centered beforehand so it needn t to adjust in the use process If the ring is not in the center you could adjust by the centering bolt The 10X 20X light ring is worked with the 10X 20X phase contrast objective while the opening is used for...

Страница 20: ...ter of the light path 3 When in the phase contrast observation do keep the aperture diaphragm adjustment lever on the position of O wide opening 5 2 2 The centering ring fig 29 and fig 30 Usually you needn t the operation of centering If necessary please accord to the following steps 1 Place the specimen on the stage and focus it 2 Take out the eyepiece replace it with the CT the centering telesco...

Страница 21: ...ce of the light ring center and the phase contrast center when changing the objective If having departure you ought to center again If the light ring is centering incorrectly you will fail to obtain the best viewing effect of the microscopy After removing or replacing a thick specimen the light ring and the phase contrast ring are likely to deviate each other which will result in a decline of the ...

Страница 22: ...n the locking bolt on the trinocular viewing tube and take out the dust cap 2 Remove the dust cover on the both ends of the video accessories and revolve the screw head end into the CCD CMOS port 3 Install the accessories into the tri through port and screw down the bolt 6 1 3 Focus fig 32 Doing a binocular observation at 20 brightness look the image on the video or the computer which connected wi...

Страница 23: ...lug the digital photo connected head into the photo tube then screw down the locking bolts Before connecting the camera and adapter remove the camera lens firstly then connect the lens port with the adapter Pay attention to the gate type please To avoid the disturbing from the ocular in the observation please place the viewer finder on the two sides of the microscope when installing the camera set...

Страница 24: ...o focus the specimen Please refer the user manual of the camera set to obtain the details 6 2 4 Adjusting the color temperature When shooting the chromophotograph please use the sunlight film 1 Mount the LBD temperature changed color filter on to the color filter bracket 2 Turn the brightness adjustment knob to the maximal position so you obtain a sunlight illumination ...

Страница 25: ...m the surface focus of stage plate up 8mm down 3mm Stage Area 160 width 250 Length mm Mechanical ruler Movement Range 120 width 78 Length mm Illumination Halogen Lamp 6V30W Preset Center Intensity continued Adjustable Condenser Long working Distance Condenser Numerical Aperture 0 3 Working Distance 72mm Operation environment Use indoor Altitude Maximum 2000 m Temperature 5 40 41 F 109 F Maximum Re...

Страница 26: ...lters 11 No use the appointed lamp bulb use the specified halogen Lamp 6V30W 3 2 The edge of the field of view has shadow or the brightness is asymmetry The nosepiece is not in the located position Turn the nosepiece into the position where you can hear clicked 4 the color filter is stopped midway 5 y l p e e d t r e s n I The phase contrast slider is not located in the proper position Turn the sl...

Страница 27: ...e contrast kits is not centered Adjust the bolts to center them 12 The objective used is not fit to the phase contrast observation Please use the compatible objective 12 When looking at the edge of the culture dish the phase contrast ring and the light ring is deviated each other Moving the culture dish until you obtain the phase contrast effect You also could demount the slider and dail the field...

Страница 28: ... brightness adjustment knob is used wrong Adjust the brightness adjustment knob in a correct way 8 2 The light glimpse The bulb is going to spoil Change the bulb 3 The power cord have a poor contact Check the power cord and connect them exactly 6 Ⅳ Viewing tube The two eyes field of view is different The interpupillar distance is not correct Adjust the interpupillar distance 10 The diopter is not ...

Страница 29: ...al Limited objective 3 The indoor window or the fluorescence lamp develop The extra light entered into the eyepiece and viewfinder is reflected Cover up the eyepiece and the viewfinder of the microscope illumination system ...

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