Biotek Synergy HTX, Руководство оператора

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Chapter 5: As-Needed Maintenance
8.
Reassemble the instrument as necessary.
9.
If the dispense module is installed, detach the outlet tubes from the rear panel
of the instrument. If it is not installed, attach just the dispense module’s
communication cable to the instrument. Remove the supply bottles and their
holders.
10.
Perform the procedures described below through page 88 to decontaminate the
fluid lines in the dispense module, the internal tubing and injector heads, and
the tip priming trough and priming plate.
Decontaminate the Fluid Lines
1.
Place a beaker with 20 mL of 0.5% sodium hypochlorite solution
or
70%
isopropyl alcohol near SYRINGE 1 on the dispense module.
2.
Place the SYRINGE 1 inlet tube in the beaker.
3.
If you have not already done so, detach the dispense module’s outlet tubes
from the instrument’s rear panel. Place the ends of the outlet tubes in an empty
beaker and set the beaker on the work surface.
4.
Launch Gen5, select
System > Instrument Control,
and click the
Prime
tab.
5.
Select
Dispenser 1
, enter a Volume of
5000 µL
, and keep the default dispense
Rate.
6.
Place the priming plate on the carrier (it is not used, but the reader requires its
presence).
7.
Run two prime cycles, for a total of 10000 µL.
8.
Pause for 20 to 30 minutes to allow the solution to disinfect the tubing.
9.
Remove the inlet tube from the beaker of disinfectant solution.
10.
From the Reader Control dialog, change the Volume to
1000 µL.
11.
Run one prime cycle, to flush the disinfectant out of the fluid lines.
12.
Empty the beaker containing the outlet tubes. Put the tubes back in.
13.
Important! If sodium hypochlorite (bleach) was used, perform
Rinse the Fluid
Lines
on the next page.
Otherwise, (or after performing the Rinse procedure), repeat steps 1–13 for
SYRINGE 2 / Dispenser 2.
BioTek Instruments, Inc.