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800™ TS

Absorbance Reader

Instructions for Use

BioTek

Instruments, Inc.

PN 156

1011

, Rev A

Содержание 800 TS Series

Страница 1: ...800 TS Absorbance Reader Instructions for Use BioTek Instruments Inc 2017 PN 1561011 Rev A ...

Страница 2: ...of BioTek Instruments Inc BioCell is a trademark of BioTek Instruments and is patented under U S patent number 5 963 318 Microsoft Windows and Excel are either registered trademarks or trademarks of Microsoft Corporation in the United States and or other countries All other trademarks are the property of their respective holders Restrictions and Liabilities Information in this document is subject ...

Страница 3: ...ormation For customer service sales and technical assistance refer to the information below Customer Service and Sales Internet www biotek com Phone 888 451 5171 toll free in the U S 802 655 4740 outside the U S Fax 802 655 7941 Email customercare biotek com Service Technical Assistance Center TAC Phone 800 242 4685 toll free in the U S 802 655 4740 outside the U S Fax 802 654 0638 Email tac biote...

Страница 4: ... include the confirmation that performance charactertistics for the specific assay s are met Quality Control It is considered good laboratory practice to run laboratory samples according to instructions and specific recommendations included in the assay package insert for the test to be conducted Failure to conduct Quality Control checks could result in erroneous test data Warnings Operate the ins...

Страница 5: ...icant on the carrier mechanism or components in the carrier compartment will attract dust and other particles which may obstruct the carrier path and cause the instrument to produce an error Warning Liquids Avoid spilling liquids on the instrument fluid seepage into internal components creates a potential for shock hazard If a spill occurs while a program is running abort the program and turn off ...

Страница 6: ...es on internal components Caution Spare Parts Only approved spare parts should be used for maintenance The use of unapproved spare parts and accessories may result in a loss of warranty and potentially impair instrument performance or cause damage to the instrument Caution Touchscreen Do not use sharp implements to operate the touchscreen Using a sharp stylus or other implement may damage the disp...

Страница 7: ... Failure to follow maintenance protocols may void the warranty See Chapter 4 Maintenance Caution Electromagnetic Environment Per IEC 61326 2 6 it is the user s responsibility to ensure that a compatible electromagnetic environment for this instrument is provided and maintained in order that the device will perform as intended Caution Electromagnetic Compatibility Do not use this device in close pr...

Страница 8: ...c Discharge EN 61000 4 3 Radiated EM Fields EN 61000 4 4 Electrical Fast Transient Burst EN 61000 4 5 Surge Immunity EN 61000 4 6 Conducted Disturbances from RFI EN 61000 4 11 Voltage Dips Short Interruptions and Variations Directive 2014 35 EU Low Voltage Safety The system has been type tested by an independent testing laboratory and was found to meet the requirements of this Directive Verificati...

Страница 9: ...environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at their own expense In order to m...

Страница 10: ...ng UnderwritersLaboratoriesUL61010 1 Safetyrequirementsforelectrical equipment for measurement control and laboratory use Part 1 General requirements CanadianStandardsAssociationCAN CSAC22 2No 61010 1 Safety requirements forelectricalequipmentformeasurement controland laboratoryuse Part1 General requirements EN 61010 Standards see CE Mark starting on page viii ...

Страница 11: ...hing or pinching Attention risque d écrasement et pincement Warnen Gefahr des Zerquetschens und Klemmen Precaución riesgo del machacamiento y sejeción Attenzione rischio di schiacciare ed intrappolarsi Warning hot surface Attention surface chaude Vorsicht heiße Oberfläche Precaución superficie caliente Attenzione superfice calda Laser radiation Do not stare into beam Rayonnement laser Ne pas regar...

Страница 12: ...xvi Safety Symbols BioTek Instruments Inc ...

Страница 13: ...Installation ...

Страница 14: ...euse if the instrument needs to be shipped again Save all packaging materials If you need to ship the reader to BioTek for repair or replacement you must use the BioTek supplied materials Using other forms of commercially available packaging or failing to follow the repackaging instructions may void your warranty During the unpacking process inspect the packaging reader and accessories for shippin...

Страница 15: ... Remove the six black screws holding the cover to the base then lift the base off the cover and set it right side up on a level surface 3 Remove the two screws on each of the shipping brackets remove the brackets and store the screws in the holes on the brackets Remove and store all shipping hardware before you turn on the reader ...

Страница 16: ...Verify the Filter Wheel Contents The 800 TS ships with up to five preordered filters in the filter wheel During installation it is good practice to verify the filters and their placement and to confirm that the software filter table matches the filter wheel s configuration discussed later All five locations in the wheel must contain either a filter or a plug Before continuing obtain a clean lint f...

Страница 17: ...cess the filter wheel 1 With the top case removed from the reader see Remove the Shipping Hardware on page 3 for directions remove the four thumbscrews from the optic arm and lift the optic arm cover off if equipped 2 Disconnect the two connectors on the back of the incubation housing then gently lift the housing off of the reader ...

Страница 18: ...d in the filter wheel and the location of each filter You will use this information later to verify edit the software filtertable Compare the filters with your model s default filters or with the purchase order if different filters were ordered Contact BioTek Customer Care if you did not receive the expected filters plugs Each filter is labeled with a wavelength and an arrow showing the light dire...

Страница 19: ...p cover by replacing the six screws 4 Select an Appropriate Location Install the reader on a level stable surface Select an area where ambient temperatures between 18 C 71 6 F and 40 C 104 F can be maintained The reader is sensitive to extreme environmental conditions Avoid the following Excessivehumidity Condensationdirectlyonthesensitiveelectroniccircuitscan causetheinstrumenttofailinternalself ...

Страница 20: ... creating a severe shock hazard Always connect the system power cord directly to an appropriate receptacle with a functional ground a Connect the power cord to the external power supply b Locate the power inlet on the rear of the reader c Plug the rounded end of the power supply s cord into the power inlet d Plug the other end of the power cord into an appropriate power receptacle 6 Optional Insta...

Страница 21: ...ight side of the instrument and turn on the reader The reader delays any action until the bulb has warmed up three minutes for narrow beam and UV models and 30 seconds otherwise Then the reader performs a power up system test 2 When the system test is completed the touchscreen displays its main screen Refer to the instructions that shipped with the Gen5 software to install the necessary drivers Th...

Страница 22: ...r value and use the keypad to enter the correct time for both the hour and minutes then click OK Note You can change just the minutes value Tap the minutes value and enter the correct time 3 Tap the Date button 4 Tap month day or year and use the keypad to set the current date Tap OK when done 11 Verify the Reader s Filter Table Before using the 800 TS verify that the filter table reflects the fil...

Страница 23: ...ges tap Save in the Installed Filters screen The filter values are now available for selection in protocols and the absorbance test plate setupscreen 12 Optional Establish Communication 1 On the host computer start Gen5 and log in if prompted The default System Administrator password is admin 2 From the Gen5 main screen select System Instrument Configuration and click Add 3 Set the Reader Type to ...

Страница 24: ...all the USB driver software Is the touchscreen at the Main Menu 13 Run a System Test Running a system test will confirm that the reader is functioning properly or will provide an error code if a problem is detected Using the Touchscreen 1 If the reader is equipped with an incubator turn it on a On the Main Menu tap the temperature display circled in the following image When the incubator is turned...

Страница 25: ...e Quick Menu screen turn on the Temperature Control and enter a setpoint of at least 37 C then tap Home to return to the Main Menu c Wait for the temperature display to reach the defined setpoint before continuing 2 From the Main Menu tap Instrument Options ...

Страница 26: ...er select 800 TS and click OK 3 If a message appears stating that the reader has a pending system test report click OK then click Close Again select System Diagnostics Run System Test If prompted to select a reader select 800 TS and click OK 4 When the test is completed a dialog requesting additional information appears Enter the information and click OK 5 The results report appears and should con...

Страница 27: ... design is subject to change If the instructions in this section do not appear to apply to the packaging materials you are using please contact BioTek s Technical Assistance Center for guidance Replace the shipping hardware before repackaging the instrument Please contact BioTek if you need to replace any of these items Carrier shipping bracket PN 1560517 Carrier shipping bracket screws PN 19668 S...

Страница 28: ...BioTek Instruments Inc 24 Repackaging and Shipping Instructions ...

Страница 29: ...Getting Started ...

Страница 30: ...re provided via the touchscreen With optional Gen5 software kinetic and well area scanning read modes are supported along with BioCell and 60 72 and 96 well Terasaki plates Gen5 also offers extensive data analysis and reporting and exporting capabilities The reader is available in five models The filter wheel contains up to five filters and is user accessible For models with fewer than five filter...

Страница 31: ... 27 800 TS External Components 1 Microplate carrier 2 Touchscreen 3 Power switch 4 USB flash drive port for USB flash drive Note that the photo does not show the door ...

Страница 32: ...on a reader without incubation capability 1 Power port 2 USB port 3 DO NOT USE For BioTek Service only 4 USB port for printer Rear ports on a reader with incubation capability 1 Power port 2 USB port 3 DO NOT USE For BioTek Service only 4 USB port for printer ...

Страница 33: ...vate a tab tap the item once To return to the Main Menu from any other screen tap Home in the top left corner For instructions on cleaning the touchscreen see the Maintenance chapter Note Topreserve thelife ofthebulb turnit off whennotneededbytapping in the Instrument or Quick Menu Note that if you turn off the bulb the reader will go throughthebulbwarm upprocesswhenyouturnthebulbbackon threeminut...

Страница 34: ...anddefineblankwell s Ifapplicabletoyourreader mode shakeoptions are available Results Viewandoptionallyprint exportmeasurementdatastoredonthe reader forthe12mostrecentlyrunprotocols Instrument Configure thereader printer andUSBflashdrive settings define results outputcriteria runanAbsorbancePlateTest andmore Configure Your 800 TS After you install the reader and before you use it to create and run...

Страница 35: ...ime for both the hour and minutes then click OK Note You can change just the minutes value Tap the minutes value and enter the correct time 3 Tap the Date button 4 Tap month day or year and use the keypad to set the current date Tap OK when done Verify or Change the Reader s Filter Table Important The reader s filter table must exactly match the contents of the installed filter wheel ...

Страница 36: ...n the Installed Filters screen The filter values are now available for selection in protocols and the absorbance test plate setupscreen Define Regional Settings 1 From the Main Menu tap Instrument the Config tab and then Regional Settings 2 Tap to togglebetween Time format AM PM or 24 hour Date format mm dd yyyy or dd mm yyyy Decimal symbol period or comma Note This setting does not apply to the s...

Страница 37: ...alues asapplicable ThisfilecanbeopenedinExcelorotherspreadsheet software Gen5 Input Generates a text file that can be opened in Gen5 using the Read from File option This file contains only raw data not delta ODs or blanks See page 92 for sample CSV and text files Define and Start a Quick Protocol 1 From the Main Menu tap Quick 2 In the Quick Menu set the Primary and if applicable Secondary wavelen...

Страница 38: ...with each read speed type in the Specifications appendix Note that Sweep is not available on the NB model 6 Toggle to set the plate type 6 12 24 48 96 or 384 wellmicroplates Note Only full plate reads are supported on the touchscreen To read a partial plate you must use Gen5 7 If applicable tap the Blank tab to add blank wells a In the Blank Well screen use the keypad to enter which well should be...

Страница 39: ...1 From the Main Menu tap Protocol 2 Tap the protocol that you want to modify then tap Edit 3 Make any desired changes then tap Save For instruments equipped with incubation capability To perform incubation during measurement either turn on incubation from the Incubate tab Quick Menu or tap the temperature to the left of the Run Protocol in a defined protocol The onboard storage for the touchscreen...

Страница 40: ...s The results are displayed on the touchscreen 2 Tap Output The results are printed and or saved to the USB flash drive depending on the output format you selected Operate the Reader Using Gen5 Software BioTek Gen5 software supports all 800 TS reader models Use Gen5 to control the reader perform data reduction and analysis on the measurement values print or export results and more This section pro...

Страница 41: ...Maintenance ...

Страница 42: ...res Warning Internal Voltage Turn off and unplug the instrument for all maintenance and repair operations Important Do not immerse the instrument spray it with liquid or use a dripping wet cloth on it Do not allow water or other cleaning solution to run into the interior of the instrument If this happens contact the BioTek Service Department Do not soak the touchscreen This will cause damage Moist...

Страница 43: ...ree cotton cloths 1 Turn off and unplug the instrument 2 Wet a clean cotton cloth with water or with water and mild detergent then thoroughly wring out the cloth so that liquid does not drip from it 3 Wipe the plate carrier the inside of the plate carrier door if equipped and all exposed surfaces of the instrument 4 If detergent was used wipe all surfaces with a cloth moistened with water 5 Use a ...

Страница 44: ...ory instrument that has been used for research or clinical analysis is considered a biohazard and requires decontamination prior to handling Decontamination minimizes the risk to all who come into contact with the instrument during shipping handling and servicing Decontamination is required by the U S Department of Transportation regulations Persons performing the decontamination process must be f...

Страница 45: ...d Materials Sodium hypochlorite NaClO or bleach 70 isopropyl alcohol as an alternative to bleach Deionized or distilled water Safety glasses Surgical mask Protective gloves Lab coat Biohazard trash bags 125 mL beakers Clean lint free cotton cloths Procedure 1 Turn off and unplug the reader from the power supply 2 Prepare an aqueous solution of 0 5 sodium hypochlorite NaClO or bleach If the effects...

Страница 46: ...e surfaces clean from fingerprints and debris by simply wiping with a lens tissue or other lint free cloth When changing or replacing filters it is critical that the filters be placed in the filter wheel in the correct orientation with the light direction arrow pointing downward Also the reader or Gen5 software filter table must exactly match the contents of the filter wheel 1 Use a clean dry lint...

Страница 47: ...r replacing filters it is critical that the filters be placed in the filter wheel in the correct orientation with the light direction arrow pointing downward Also the reader or Gen5 software filter table must exactly match the contents of the filter wheel ...

Страница 48: ...BioTek Instruments Inc 44 Filter Storage and Handling ...

Страница 49: ...Instrument Testing ...

Страница 50: ...pecifications in the UV range 340 nm Define the Absorbance Test Plate Parameters Using the Touchscreen 1 Obtain the current Test Plate Calibration Certificate 2 From the Main Menu tap Instrument then tap Test Plate 3 Tap Test Plate Certificate and enter the plate s serial number and Next Calibration Due date then tap Back to return to the Test Plate tab 4 The wavelength selection buttons reflect t...

Страница 51: ...een click System Diagnostics Test Plates Run If prompted select the desired Test Plate and click OK 2 When the Absorbance Test Plate Options dialog appears enter any required information 3 Highlight the wavelength s to be included in this test 4 Optional Enter a comment 5 Click Start Test 6 Place the Absorbance Test Plate on the microplate carrier with well A1 in the proper location 7 Click OK to ...

Страница 52: ...d shake well The solution should measure approximately 2 000 OD when using 200 µL in a flat bottom microwell Solution B Deionized water FD C Yellow No 5 dye powder typically 90 pure Tween20 polyoxyethylene 20 sorbitanmonolaurate orBioTekwettingagent PN 7773002 a 10 Tween solution Precision balance with capacity of 100 g minimum and readability of 0 001 g Liter volumetric flask Weigh boat 1 Weigh o...

Страница 53: ... it to an Excel spreadsheet Calculations Absorbance Liquid Test 1 Accuracy Specification 1 0 0 010 OD from 0 000 to 2 000 OD Repeatability Specification 0 5 0 005 OD from 0 000 to 2 000 OD 1 The plate is read five times in the Normal position at 405 nm Calculate the Mean OD and Standard Deviation of those five reads for each well in columns 1 and 2 2 For each well in columns 1 and 2 calculate the ...

Страница 54: ...on at 405 nm Calculate the Mean OD of those five reads for each well in columns 11 and 12 4 Perform a mathematical comparison of the Mean values for each well in its Normal and Turnaround positions that is compare A1 to H12 A2 to H11 B1 to G12 H2 to A11 To pass the test the differences in the compared Mean values must be within the Accuracy specification for a 96 well microplate Example If the Mea...

Страница 55: ...Specifications ...

Страница 56: ...en gas filled bulb 800TSUV 800TSUVI 800TSNB models Halogen bulb Dimensions 16 5 D x 15 W x 7 H 41 9 x 38 1 x 17 8 cm Weight 22 lbs 10 kg Environment Operational temperature range 18 to 40 C Humidity 10 to 85 relative humidity non condensing Power Supply 24 volt DC power supply compatible with 100 240 V AC 50 60 Hz Power Consumption 40W maximum non incubated models 150W maximum incubated models Inc...

Страница 57: ...0 010 OD from 0 000 to 2 000 OD 405 nm Rapid Read Mode 2 0 0 020 OD from 0 000 to 2 000 OD 405 nm Sweep Read Mode 1 0 0 020 OD from 0 000 to 1 000 OD 405 nm Linearity Normal Read Mode 1 0 0 010 OD from 0 000 to 2 000 OD 405 nm 3 0 0 010 OD from 2 000 to 3 000 OD 450 nm Rapid Read Mode 2 0 0 010 OD from 0 000 to 2 000 OD 405 nm Sweep Read Mode 1 0 0 010 OD from 0 000 to 1 000 OD 405 nm Repeatabilit...

Страница 58: ...d Read Mode 400 nm 750 nm 0 000 to 4 000 OD Sweep Read Mode 400 nm 750 nm 0 000 to 3 000 OD Absorbance Resolution 0 001 OD when operated in standalone mode 0 0001 OD when operated with Gen5 Accuracy 340 nm to 399 nm Normal Read Mode 2 0 0 010 OD from 0 000 to 2 000 OD 340 nm Rapid Read Mode 2 5 0 020 OD from 0 000 to 2 000 OD 340 nm Accuracy 400 nm to 750 nm Normal Read Mode 1 0 0 010 OD from 0 00...

Страница 59: ...00 nm to 750 nm Normal Read Mode 0 5 0 005 OD from 0 000 to 2 000 OD 405 nm Rapid Read Mode 1 0 0 010 OD from 0 000 to 2 000 OD 405 nm Sweep Read Mode 2 0 0 020 OD from 0 000 to 1 000 OD 405 nm Throughput From carrier start to carrier stop 96 Well Single Wavelength Normal Read Mode 39 seconds 96 Well Dual Wavelength Normal Read Mode 73 seconds 96 Well Single Wavelength Rapid Read Mode 26 seconds 9...

Страница 60: ...d Read Mode 96 well 2 0 0 010 OD from 0 000 to 2 000 OD 405 nm Normal Read Mode 384 well 2 5 0 010 OD from 0 000 to 2 000 OD 405 nm Rapid Read Model 384 well 2 5 0 010 OD from 0 000 to 2 000 OD 405 nm Repeatability STD Normal Read Mode 96 well 0 5 0 005 OD from 0 000 to 2 000 OD 405 nm Rapid Read Mode 96 well 1 0 0 010 OD from 0 000 to 2 000 OD 405 nm Normal Read Mode 384 well 1 5 0 010 OD from 0 ...

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