Biotage Isolera Four, Руководство пользователя

Страница: 104 / 116

411829-L, Collection and Fractionation April 2012 Page A-1
Appendix
A
Collection and Fractionation
This appendix describes the details of the different collection and fractionation methods that are
available for the Isolera system. It also contains information on how gradients are automatically
extended.
A.1 Collection and Fractionation Methods
The Isolera system supports several collection and fractionation methods controlled by the internal
detector or on an external signal. The internal detector provides collection and fractionation
methods based on one or two specific wavelengths, or on a continuous wavelength range. It is
possible to combine these methods in a number of ways as described in section A.1.4.
A.1.1 Collection Methods
The following collection methods are supported:
• Manual : Collection is started and stopped by the user.
• Collect All : Everything is collected.
• Threshold : Everything above a specified light absorption threshold level is collected.
• Slope : Collection is started when the slope of the light absorption is above a specified limit
and stopped when the absorption level is below the level detected when the collection was
started. Note that the Slope collection mode is not available with the
λ -all detection mode or
with an external detector.
A.1.2 Fractionation Methods
The following fractionation methods are supported:
• Manual : Manual fractionation.
• Volume : Fractionation when a test tube or bottle is full (based on the specified max fraction
volume for the vessel type).
• Valley : Fractionation when a valley is detected in the light absorption curve.
• Shoulder : Fractionation when a shoulder is detected in the light absorption curve.
A.1.3 Detector Signals
The Isolera system includes either a fixed UV detector using the wavelength 254 nm, a dual
variable UV detector with the range 200-400 nm, or a UV-VIS detector with the range 200-800 nm.
(The UV-VIS detector is not available with Isolera Prime and the fixed UV detector is not available
with Isolera LS.) It is also possible to connect an external detector to the system, e.g. an Biotage
ELSD-1080 detector. Depending on the detector in use, collection and fractionation are performed
slightly different.
• Fixed UV detector : The system uses the single signal for both collection and fractionation.
• Dual UV or UV-VIS detector: The dual detectors have four different detection modes:
1. UV1: The system uses the UV1 signal for both collection and fractionation. The UV2
signal is used only for monitoring of the progress of the purification.
2. UV1+UV2: The system uses both the UV1 and UV2 signal for collection and fractionation.
3. λ -all: The system uses average absorbance within a user-defined range for collection and
fractionation. (Only available on systems with a Spektra license installed.)