Biotage Isolera Four, Руководство пользователя

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411829-L, Troubleshooting April 2012 Page 6-5
6
6.7 Leak Detected
See “Leaks” on page 5-7.
6.8 Overpressure Detected
Possible Causes Solutions
1. Blockage due to kinked
tubing.
a. Press Purge... in the alarm dialog.
b. When the pressure has been reduced to an acceptable level, press End in
the Purge dialog.
c. Press Pause in the alarm dialog.
d. Shut down the system; see page 4-1. (To be able to shut down the
system, you have to abort the task in progress.)
e. Straighten or replace the kinked tubing. For more information, see
“Assembling Tubes Correctly” on page 5-9.
WARNING
To avoid being struck by the collection arm, keep your
hands out of range of the collection arm while the
homing routine runs in step f below.
f. Turn on the system. The collection arm moves through its homing routine
and the system boots to the system’s main menu.
2. The flow rate is too high
for the purification or
prime.
a. Press Purge... in the alarm dialog.
b. When the pressure has been reduced to an acceptable level, press End in
the Purge dialog.
c. Press Pause in the alarm dialog.
d. If the overpressure occurred during a prime: a) Press End Prime at the
Prime tab and start a new prime with a lower flow rate.
e. If the overpressure occurred during an equilibration or a gradient run:
a) Press Edit... at the Status tab. The Edit Method dialog opens.
b) Lower the flow rate and press OK .
c) To resume the run, press Resume in the right-hand panel.
3. Blockage in the
cartridge, tubing, or
detector flow cell from
precipitations.
Note that there may be
precipitation in areas
not visible.
a. Press Purge... in the alarm dialog.
b. When the pressure has been reduced to an acceptable level, press End in
the Purge dialog.
c. Press Pause in the alarm dialog.
d. Shut down the system; see page 4-1. (To be able to shut down the
system, you have to abort the task in progress.)
e. Visually inspect all tubing for precipitation. If found, remove and clean the
tubing. For more information, see “Assembling Tubes Correctly” on
page 5-9.
f. Visually inspect the detector flow cell for precipitation. If found, clean the
flow cell. For more information, see page 5-3.
WARNING
To avoid being struck by the collection arm, keep your
hands out of range of the collection arm while the
homing routine runs in step g below.
g. Turn on the system. The collection arm moves through its homing routine
and the system boots to the system’s main menu.
h. Prime the system using e.g. methanol; see page 4-33.