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User Manual (Ver. 1.1)

ExiPrep™16 Plus

Fully Automated Nucleic Acid Extraction System

Содержание A-5030

Страница 1: ...User Manual Ver 1 1 ExiPrep 16 Plus Fully Automated Nucleic Acid Extraction System...

Страница 2: ...7 V DNA RNA Extraction 9 1 Contamination shield installation 9 2 Sample Preparation 10 3 RUN 12 VI ExiPrep 16 Plus Setup 15 1 Main Menu 15 2 Registering a New User 19 3 Viewing Run History 20 4 Managi...

Страница 3: ...rmation about ExiPrepTM 16 Plus You can download up to date product information and new protocols General information ExiPrep 16 Plus is a trademark of Bioneer Corporation The information contained in...

Страница 4: ...instrument is stopped either from operator error including improper accessory insertion or manually halting the instrument during normal operation you must re initialize the instrument before pulling...

Страница 5: ...act results 3 Operate the instrument within the recommended humidity range 20 80 no condensation Humid conditions may cause corrosion or malfunction 4 Do not place any objects next to or behind the in...

Страница 6: ...pulled out while the Heating Block is not in its normal initialized position the interference in movement can cause Heating Block and other internal component damage and lead to instrument malfunction...

Страница 7: ...nents and Specifications 1 System Components Part Name Cat No Qty Check ExiPrep 16 Plus A 5030 1 ea User s manual 1 ea Waste tray 1 ea Elution tube rack 1 ea Disposable tip rack 1 ea Setup tray 1 ea C...

Страница 8: ...mm 320 W x 487 H x 535 D Weight 22 kg Operating temperature 15 30 C Operating humidity 20 80 no condensation Operating system Stand alone Electrical Voltage Frequency 100 240 V 50 60 Hz Network suppor...

Страница 9: ...door and remove the tie wrap from the belt Make sure that all components are included Refer to the list of components on page 5 1 Open the door and remove the Screw bolt A from the rail 2 Remove the P...

Страница 10: ...n 7 Press the power button on the LCD screen to initialize the instrument A progress bar on the lower portion of the LCD touchscreen will indicate initialization progress 8 Open the door and remove th...

Страница 11: ...nation shield CAUTION In order to use contamination shield this must be installed on the Base plate accessory 2 Place the contamination shield on the lower right side of the Syringe block Contaminatio...

Страница 12: ...the DNA RNA extraction kits sold separately 3 Make sure you have purchased the correct kit for the sample type and target nucleic acid type you desire Insert the desired number of Elution tubes E int...

Страница 13: ...aling the film of Buffer Cartridge using the 6 Hole punch tool in the same pattern as Buffer Cartridge 7 Load samples into the Sample Loading Well of Buffer Cartridge 8 Preparation for DNA RNA extract...

Страница 14: ...id and sample source type 3 Verify the Prep Type and Sample SRC of the three digit code you have entered 4 Press the Enter button to access the elution volume selection menu 5 Select the elution volum...

Страница 15: ...ake sure the Buffer Cartridge fits snugly and exactly into its place Misplacement of the Buffer Cartridge may result in instrument break down and malfunction 8c Place the Elution tube rack on the Base...

Страница 16: ...the Base Plate and remove the Elution tubes Buffer Cartridges and all racks from the Base Plate After removing all accessories push the Base Plate back in completely and close the door 12 You are giv...

Страница 17: ...s UV lamp ON OFF selection menu Sterilize the internal cavity with the built in UV lamp Press the UV lamp button to begin sterilization The sterilization runs for 5 minutes To cancel press the UV lamp...

Страница 18: ...SETUP MENU screen PREP SETUP screen Selecting Start from the Main Menu will bring up the PREP SETUP screen where you can enter the three digit code for the extraction and sample source type Refer to t...

Страница 19: ...ress the UV lamp icon to initiate the UV sterilization process The icon will turn yellow as UV sterilization proceeds The sterilization runs for 5 minutes The progress can be tracked through the progr...

Страница 20: ...ing features such as UV sterilization and system preferences Details on system configuration can be found in page 23 History Enabled by selecting the user login option Allows you to audit up to 99 mos...

Страница 21: ...ess the Setup button to access the SETUP menu 2 Press the User button to access the User Registration menu 3 Enter a 6 digit user ID using the keypad on the LCD touch screen and press Enter to save th...

Страница 22: ...ory 1 Press the Setup button to access the SETUP menu 2 Press the History button to view the instrument run history 3 The run history contains the following parameters No Recent runs have a lower numb...

Страница 23: ...button to access the SETUP menu 2 Press the Config button to access the System Setup menu 3 Press the User button to enable login mode If the login mode is enabled a popup prompt User Mode ON appears...

Страница 24: ...ter every DNA RNA extraction run 1 Press the Setup button to access the SETUP menu 2 Press the Config button to access the System Setup menu 3 Press the UV lamp button to enable automatic UV steriliza...

Страница 25: ...Screen Calibrates the screen position Calibrate the screen position relative to touching Press and hold the circle at the upper left corner with a blunt tool for 2 seconds Press and hold the circle a...

Страница 26: ...LCD touchscreen 3 Press the Enter button 4 The Admin Menu screen includes an option to delete user IDs or change the administrator ID Select User list delete 1 to delete unused IDs Select Administrat...

Страница 27: ...r ID change menu Enter a new 6 digit administrator ID using the keypad in the middle of LCD touch screen and press Enter button to save Press the ok button to save new administrator ID after verifying...

Страница 28: ...ing to the following instructions The default IP address for ExiPrepTM 16 Plus is 10 10 104 88 1 Execute the program 2 Click the IP Select button on the lower left corner 3 A new window IP Machine Sel...

Страница 29: ...e right side Wait until the progress bar is full If the connection stops progressing restart the update process from step 1 9 Once the connection process is done Memory Check starts window will pop up...

Страница 30: ...ick the connection and select Properties iii Select Internet Protocol TCP IP iv Select Use the following IP address v Enter the IP address subnet mask Default gateway and DNS server address informatio...

Страница 31: ...m 1 14 Genomic DNA Buffy coat 2 14 Total RNA Buffy coat 1 15 Genomic DNA Sputum 2 15 Total RNA Sputum 1 16 Genomic DNA BAL 2 16 Total RNA BAL 1 17 Genomic DNA Saliva 2 17 Total RNA Saliva 1 18 Genomic...

Страница 32: ...DNA Serum 3 14 mRNA Buffy coat 4 14 viral DNA Buffy coat 3 15 mRNA Sputum 4 15 viral DNA Sputum 3 16 mRNA BAL 4 16 viral DNA BAL 3 17 mRNA Saliva 4 17 viral DNA Saliva 3 18 mRNA Swab 4 18 viral DNA Sw...

Страница 33: ...oat 6 14 viral DNA RNA Buffy coat 5 15 viral RNA Sputum 6 15 viral DNA RNA Sputum 5 16 viral RNA BAL 6 16 viral DNA RNA BAL 5 17 viral RNA Saliva 6 17 viral DNA RNA Saliva 5 18 viral RNA Swab 6 18 vir...

Страница 34: ...tem 32 continue No Target Sample source No Target Sample source 7 01 Plasmid DNA endA strain 9 01 Protein His Tag 7 02 Plasmid DNA endA strain No Target Sample source 8 21 Fragment DNA Gel slice 8 22...

Страница 35: ...from your dealer Power turns on but the LCD screen is black 1 Since it is most likely an instrument issue request service from your dealer Pressing the RUN button fails to start instrument operation 1...

Страница 36: ...the end of the Base Plate Slide Rail 4 Request service from your dealer The Base Plate does not slide out completely 1 Visually inspect inside the instrument for accessories and or foreign materials t...

Страница 37: ...to see if the correct protocol was selected 2 Inspect the instrument for foreign materials or accessories impeding normal movement of the Syringe Block which causes motor malfunction 3 Repeat the same...

Страница 38: ...top usage of the instrument 2 Request service from your dealer The UV Lamp does not work 1 Verify that the front door is completely closed 2 Request service from your dealer Protocol dose not update 1...

Страница 39: ...s and broken plastic on ports b to damage caused by accident abuse misuse flood fire earthquake or other external causes c to a product or part that has been modified in any way without written permis...

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