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SECTION 4.   ORDERING INFORMATION

A. The Gradient Master™ gradient forming system.

MagnaBase System: Catalog number

Model 108 Gradient Master base unit .................................

108-1 (100-120V)

108-2 (220-240V)

Includes: 4" MagnaBase rotating steel plate, 2 layering cannula, fine tip marker, 2 hex wrenches, level, SD 

card and SD card reader.

Tube Holder and Cap Selection

Order either the MagnaBase (105-9XX) tube holders from the list below by finding the match for your 

centrifuge rotor. The 6-place swinging bucket tube holders are supplied with 6 plastic caps for either 

rate zonal (suffix -R) or isopycnic (-I) gradients (e.g. 105-925-R for the MagnaBase SW28 holder and a 

set of 6 rate zonal caps). The rate zonal caps leave 4 mm above the finished gradient for sample while 

the isopycnic caps leave 10 mm.  If both long and short caps are needed, use the suffix -IR ((e.g. 105-

925-IR). The 8-place vertical tube holders may also be used for swinging bucket tubes with the same 

diameter and vice versa. Prices for the vertical tube holders do not include caps, which must be 

ordered separately if you plan to use an 8-place holder for both types of rotors (see below). 

Tube holders for swinging bucket rotors (6 tube capacity).

  

Order

Tube Size  

Beckman  

Dupont/Sorvall  

Hitachi  

Kontron

 Cat. No

mm/in

105-925

25 x 89

SW32

AH-629/38

SRP28SA

TST28.38

1 x 3 1/2

SW28

RPS27-2

RPS27

105-916

16 x 102

SW32.1

AH-629/17

SRP28SA1

TST28.17

5/8 x 4

SW28.1

RPS27-3

RPS27

RPS25-3

105-914A

14 x 89

SW41Ti

TH-641

TST41.14

9/16 x 3 1/2

105-914B

14 x 95

SW40Ti

RPS40T

9/16 x 3 3/4

105-913

13 x 51

SW50.1

AH-650

RPS65T

TST55.5

1/2 x 2

SW55Ti

RPS55T-2

SW65Ti

RPS50-2

RPS40T-2

RPS40
RPS50

105-911A

11 x 60

SW60Ti

TST-60.4

RPS56T

TST60.4

7/16 x 2 3/8

105-911B

11 x 34

TLS-55

RP55S

RP55S

7/16 x 1 3/8

7

Содержание GRADIENT MASTER 108

Страница 1: ...hments and you can download them from your PC or Mac into your Gradient Master model 107 Piston Gradient Fractionator model 152 or the Gradient Station model 153 BioComp s fax number 506 455 6157 BioC...

Страница 2: ......

Страница 3: ...5 3 OPERATOR S MANUAL 2011 BioComp Instruments Inc 650 Churchill Row Fredericton New Brunswick Canada E3B 1P6 Ph 800 561 4221 506 454 6410 Fx 506 455 6157 Email dhc biocompinstruments com Home Page ht...

Страница 4: ...he plate 10 B Recalling a gradient from memory and running it 10 C Preparing and capping the gradient tubes 11 D Forming the gradients 12 E Repeated use of the same gradient 12 F Developing your own g...

Страница 5: ...NOTES and FREQUENTLY USED GRADIENTS 3...

Страница 6: ...pilled gradient solutions no user service is required The Model 108 contains a computer which should not be tampered with In the unlikely event of computer failure contact BioComp and we will ship you...

Страница 7: ...are accessible The user may enter 10 gradients of their own for any rotor Internet programmability Software upgrades for both the operating system and the gradient run parameters are independently up...

Страница 8: ...SECTION 3 PARTS DIAGRAMS 6...

Страница 9: ...m If both long and short caps are needed use the suffix IR e g 105 925 IR The 8 place vertical tube holders may also be used for swinging bucket tubes with the same diameter and vice versa Prices for...

Страница 10: ...a full set of 6 caps put the suffix 6 after the catalog number e g 105 425 6 for a set of 6 rate zonal caps for the SW28 B The Western Roller blot development system Bottles Choose from the MagnaBase...

Страница 11: ...a pair of pliers to increase the torque applied To insure that the set screw is exactly perpendicular to the flat rotate the plate slightly back and forth as you tighten so the set screw can find the...

Страница 12: ...1 EXIT The rotors you purchased tube holders for should appear If more than three rotors are loaded press the arrow key to shift the display until your rotor appears Press the key under the rotor you...

Страница 13: ...e and make mark with fine tip marker on the tube at the appropriate step in the marker block 2 Before filling the tubes place them bottom down into the appropriate Marker Block and use the fine tip ma...

Страница 14: ...ith a gentle twisting motion so that any liquid remaining in the air outlet does not disturb the gradient 7 MagnaBase holders are placed in the center of the plate Caution with the large 1 tubes an un...

Страница 15: ...vy solution be dyed so that you know when to stop mixing and so you can evaluate the gradient s shape either visually or with a flow cell We have had success with 1 2 ml 0 4 Trypan Blue 100 ml solutio...

Страница 16: ...ENTR Pressing the NEXT key will scroll through the values on the EDIT screen 5 Pressing EDIT on light and heavy will bring up the screen Short Sucr 02 28 wv ENTR Use Num Keypad EXIT 6 If you enter a n...

Страница 17: ...the order is 1 2 1 2 1 2 1 2 1 2 3 IMPORTANT the time should be entered as 0 00 for any step that you want to leave indeterminate typically the last step which is stopped manually as the blue dye star...

Страница 18: ...mediately stop it this enters it in the LAST memory Use the NEXT and DELete key to cycle through and remove unwanted memories and then enter the desired memory order S 01of01 1 NEXT DEL RUN EXIT In th...

Страница 19: ...FIXED ANGLE AND VERTICAL ROTORS USING THE ADJUSTABLE MARKER BLOCK AND QUICK SEAL TUBES I Setting the Marker Block Since the nipple tipped Quick Seal tubes can not accept a cap during mixing we supply...

Страница 20: ...a entry without changing anything and Start the run Exit and Save the run and it will appear in the VERT rotor list See Section G above for the programming instructions 3 Option 3 Manually entering a...

Страница 21: ...slot on the front panel 5 Turn the power OFF and then ON with the upper right most key pressed hold it down until you see OK BioComp Flash SDLder 1 5 LOAD MDSP GMLD Press the GMLD key The display sho...

Страница 22: ...u ll need to reload the rotor keys since loading a new Grad List erases all keys you had previously loaded Repeat the startup routine and press ROTR GRADIENT MASTER SD v1 5 NEXT Rotor SW28 LOAD Use th...

Страница 23: ...dd a shelf to gradients in large tubes containing very dense heavy solution because the shelf will already be there 4 Sample volumes are adjustable by removing solution from the top of the tube after...

Страница 24: ...t screws The Tilt motor set screw is on the grey motor base on the side of the box where the tilt motor shaft protrudes see page 6 To get clear access to it with the power off rotate the motor shaft u...

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