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C H A M B E R

www.biocare.net

U.S. patent number 6,580,056

Decloaking Chamber

™

Pro Manual

Digital programmable pressure system for heat-induced epitope retrieval

Содержание Decloaking Chamber Pro

Страница 1: ...DISPLAY SET START STOP C H A M B E R www biocare net U S patent number 6 580 056 Decloaking Chamber Pro Manual Digital programmable pressure system for heat induced epitope retrieval ...

Страница 2: ...Biocare Medical Technical Support 800 799 9499 Option 3 Customer Service 800 799 9499 Fax 925 603 8080 Corporate Office 60 Berry Drive Pacheco CA 94553 www biocare net ...

Страница 3: ...ncidental or consequential damages related to this equipment or for any warranty related services it performs Not for use with flammable solvents or gases Specifications 1 Assembly 2 Unpacking 2 Description of Parts 3 Related Parts Products 4 Operating Instructions 5 9 Setting Up 5 Recommended Protocols 5 Beginning Operation 5 Programming Display 10 Control Panel 10 Error Messages 10 Audible Alert...

Страница 4: ... is of the utmost importance for consistent immunohistochemistry IHC staining The Decloaking Chamber is designed to optimize and standardize antibody staining procedures and has been engineered to pass strict laboratory safety requirements Temperature pressure and pH can be monitored and recorded with the Decloaking Chamber to produce consistent staining which will reduce repeat testing and false ...

Страница 5: ...e returning the instrument becomes necessary You should have Lid with pressure gauge and hot sticker Gasket removable Weight petcock Pan removable Body with three prong electric cord programmable digital timer Heat shield The Decloaking Chamber is compatible with 110 120 V A step down transformer is required for laboratories using a 220 240 V electrical source When necessary the step down transfor...

Страница 6: ...in contact with the heat sensor and heat plate Vent Nozzle The top center of the chamber lid that where the weight petcock is placed Heat Shield A circular concave metal disc with perforations on it that is placed in the center of the pan Heat Sensor A circular spring loaded device within the main body that records the Actual Temperature during operation Heat Plate A large metal disc area within t...

Страница 7: ... a lighter strip The steam strip can be dated and recorded for laboratory and CAP inspections Cat No 613 C D H QC Heat Tested pH Strips The pH of retrieval solutions at high temperatures can be critical for proper IHC staining However the pH of retrieval solutions can change at high temperatures To assure quality control and consistent staining Biocare Medical has developed heat tested strips that...

Страница 8: ...sheet recommendations and protocols are based on exclusive use of Biocare products Ultimately it is the responsibility of the investigator to determine optimal conditions These products are tools that can be used for interpretation of mor phological findings in conjunction with other diagnostic tests and pertinent clinical data by a qualified pathologist Recommended protocols for HIER with Decloak...

Страница 9: ...6 Operating Instructions continued 1 2 3 Add 500 ml of DI water Place the heat shield in the center of the pan at the bottom Place the gasket into the groove on the top of the pan ...

Страница 10: ...al solution container Unused Steam Monitor Strip Steam Monitor Strip at 110 C or above for 30 seconds Place the container with the retrieval solution on top of the heat shield Do not place the container on the center of the heat shield where the heat is concentrated but towards the edge of the heat shield Do not use glass containers ...

Страница 11: ... 8 9 Place the weight petcock onto the vent nozzle Make sure there is a snug flat connection Match the word OPEN on the lid to the white dot on the handle Secure lid by turning clockwise until the word CLOSED is matched with the white dot on the handle ...

Страница 12: ...9 Operating Instructions continued Gap No Gap Correctly secured lid Gap indicates that the lid is not correctly secured ...

Страница 13: ...o be able to remove the slides The second readout is the amount of time the SP2 temperature is to be maintained before an alarm goes off The purpose of the second alarm is a quality control that allows for standardization between runs It also ensures that the slides remain inside the chamber for a standardized amount of time SP Limit readout The Set Point Limit is an internal circuit breaker that ...

Страница 14: ...to start the program Push the down arrow to display the actual temperature Both the Heat On and the Actual Temperature lights will be on When the programmed temperature is attained the Heat On light will turn off and the timer will start counting down The instrument will typically overshoot the programmed temperature by about 2 C to 4 C then settle down to the programmed temperature When the timer...

Страница 15: ...play Set button again and use the up and down arrows to set the SP Limit Note To speed up the process of setting the time push the other arrow while holding the original direction arrow down Time Bypass Feature If the SP1 time is set to 99 00 minutes the Decloaking Chamber will maintain a continuous temperature without a time countdown Temperature cannot be set higher than 100 C as a safety precau...

Страница 16: ...t the gasket for tears or wear If the vent nozzle becomes blocked with debris clear the path with a toothpick or similar device then wash clean Heat Plate and Heat Sensor Wipe off anything stuck to the heat plate or heat sensor and dry with a soft cloth Body Wipe the surface with a soft cloth soaked in warm water making sure that no water gets into the body Electrical Precautions Basic safety prec...

Страница 17: ...s lip Turn the instrument on by flipping the red toggle to the right of the control panel to the ON position Press DISPLAY SET once until the SP1 LED illuminates The user can scroll through the instrument settings by pushing the DISPLAY SET button multiple times Recommended factory settings for IHC are SP1 of 125 C for 30 seconds SP2 of 90 C for 10 seconds SP Safety Limit of 10 C unless otherwise ...

Страница 18: ...emperatures 6 Do not operate the Decloaking Chamber near a heat flame or gas source Never place the instrument directly on a stove or other heat source 7 Keep the exterior dry Remove foreign matter and water drops The heating plate sensor and the area outside of the pan must be dry and clean to work properly 8 Cool down after each use Make sure the Decloaking Chamber is cooled down after each use ...

Страница 19: ...row button Pressing the Display Set button once more will cause the SP2 temperature to display You can adjust this setting higher or lower by pressing the up or down arrow button Pressing the Display Set button once more will cause the SP2 time to display You can adjust this setting higher or lower by pressing the up or down arrow button Pressing the Display Set button once more will cause the SP ...

Страница 20: ...800 799 9499 60 Berry Drive Pacheco CA 94553 www biocare net 904 016 071817 ...

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