8
WARNINGS and Advice
•
Before
you get started for the first time on this cell sorter consult the responsible
people for proper training
•
For the moment, bacterial pathogens, cell lines or any other agent of Biosafety
level 2 or higher are not allowed, as well as radioactively labelled or flammable
samples
•
Do not forget to check the fluidics
before you start operating the cell sorter
•
Use
only MiliQ water
in the water container
•
While transferring a cap assembly to a new container, avoid touching the
assembly to the outside surfaces of the containers. If it is necessary to lay the cap
assemblies down, set only on pre-sterilized surfaces
•
It is recommended that resuspended adherent samples are mixed at high speed
to prevent clumping
•
Samples must be filtered prior to running. This will minimize clogs in the sample
tube and nozzle tip
•
Do not open
the nozzle door
•
Keep the green containment door closed as often as possible during the sorting
procedure
•
Never try to access
the laser beam paths
•
Any adjustment to the toolbars should be avoid. Only knowledgeable users must
perform alterations to the current settings. Please, refer to the responsible people for
any constraint
•
Optimal settings are automatically determined during the quality control
procedure. Adjusting any of these values will affect the sorting performance
•
Do not
adjust any setting of the global preferences menu
•
Please confirm the bead lot before starting the quality control procedure
. If the
bead lot is not in the dropdown list, please refer to the responsible people
•
Use always beads from the same lot to perform one quality control procedure
•
When the remaining run time is down to 5 minutes, the system will automatically
shut down. The fluidics will need to be refilled and the start-up procedure must be
performed. To prevent
this, perform the fluidics “hot swap”
.
