25/27
In the phase contrast
observation, the bottom
thickness of the culture dish
is more than 1.2mm.
Use a the culture dish
whose bottom thickness is
less than 1.2mm
9
Use a bright field objective
Change to the phase contrast
objective
12
The condenser ring is not
coincident with the objective
phase ring
Adjust the condenser ring to
match the objective phase
ring
12
The light ring and the phase
contrast kits is not centered
Adjust the bolts to center
them
12
The objective used is not
fit to the phase contrast
observation
Please use the compatible
objective
12
the phase
contrast effect
When looking at the edge of
the culture dish, the phase
contrast ring and the light
ring is deviated each other
Moving the culture dish
until you obtain the phase
contrast effect. You also
could demount the slider,
and dail the field diaphragm
with the direction of “
”
13
The nosepiece is not in the
center of the light path
Insure the nosepiece is in
the “clicked” position
4
The specimen don’t place
properly
Place the specimen on the
stage correctly.
9
6.one side of the
image is unfocused The optical performance of
the culture dish bottom is
poor (such as erose figure
and soon)
Please use a regular culture
dish
