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4. Using the CT to look the light ring’s image① and the phase contrast ring’s
image②, if the light ring’s image is not sharp, please shifting the CT’s
ocular until you can see a clear image of the light ring②.
5. Adjusting the bolts of the two centering holes③ in the phase contrast slider
by the screwdriver ③until the light ring center and the phase contrast center
are coincided.
6. The 10X and the 20X phase contrast objective use the same light ring on the phase
contrast slider. So you need to check the coincidence of the light ring center
and the phase contrast center when changing the objective. If having departure,
you ought to center again.
★ If the light ring is centering incorrectly, you will fail to obtain the best
viewing effect of the microscopy.
★ After removing or replacing a thick specimen, the light ring and the phase
contrast ring are likely to deviate each other, which will result in a decline
of the image contrast. So if happened, please repeat the steps as above.
★ If the container or the cover flip which used to place the specimen is not flat,
it maybe need to repeat the centering steps for obtaining a more contrast effect.
Please center the light ring by the phase contrast objective, according to the
sequence of low to high magnification.
