Making sure the device works properly
1. Connect the Pencil connector to the ThermoClamp control box (CU).
2. Connect the Pencil thermocouple plug to the Safety TC socket.
3. Connect the bath thermocouple plug to the Control TC socket (if used.)
4. Connect the tubes to their valves and reservoirs. Notice the fragile
capillaries.
5. Warning: Do not leave any solution tubes at the rear of the Perfusion
Pencil unconnected.
6. Fill and plug any unused tubes on the rear of the Perfusion Pencil with
solution to prevent backflow and cross-contamination.
7. Connect the power cable to the CU via power entry module.
8. Ground the CU using the grounding post on the rear face, and the
Pencil.
9. Turn on the on the CU power switch. The temperature controller
should display the ambient temperature. If the “Pencil Max Temp”
LED lights or the red "Safety" display shows "Err", check the Safety
thermocouple plug. If the blue "Control" temperature display is blank,
check the Control thermocouple plug (if used).
10. Adjust the setpoint above the ambient temperature. (Refer to the
section on adjusting the setpoint.) The controller will start heating
the Pencil. If the controller has not been autotuned, the temperature
will probably overshoot. (Refer to the section on autotuning the
temperature controller.)
11. To verify that the Pencil does not leak internally, open the bath
perfusion flow and all of the delivery valves. Look for leaks at each
end of the Pencil.
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