14. Inspecting this instrument
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Inspect the instrument to see if it displays the measured value correctly. Perform the inspection
periodically, e.g. once a month, also when the main unit of the instrument was subjected to a strong
impact or vibration or a measured value is different from the usual measurement. For the inspection,
use the standard sucrose solution besides purified water and the standard liquid. Standard sucrose
solutions are available from ATAGO.
Part No.
Name
RE-110010
Sucrose Solution 10%（±0.03％）
RE-110020
Sucrose Solution 20%（±0.03％）
RE-110030
Sucrose Solution 30%（±0.03％）
RE-110040
Sucrose Solution 40%（±0.04％）
RE-110050
Sucrose Solution 50%（±0.05％）
Inspection of the instrument with standard sucrose solution
1. Prepare the instrument and perform the operation before starting measurement (according to the
instruction manual).
2. Using purified water left at room temperature, perform zero adjustment correctly.
3. Set the number of measurement scale to the No.1(the scale of Brix%).
4. Measure the Brix of the standard sucrose solution (about 5 times repeatedly).
5. If the deviation of the value of the standard sucrose solution indicated by the instrument is
±0.1%, the operation of the instrument is normal. If the deviation is ±0.2% or larger, check the
purity of the sucrose, method of preparation of the sucrose solution, measuring method, etc.,
then repeat the inspection.
6. If the deviation is still ±0.2% or larger, contact your ATAGO distributor.
※How to make standard sucrose solution (20.0%)
Use the following steps to make a standard sucrose solution.
Note : Make standard sucrose solution at a temperature of 20℃±5℃.
Note : 10.0%, 30.0%, and 40.0% sucrose solutions can also be made by using the same procedure
and changing the ratios.
①Items to be prepared
•20g of sucrose of special grade
•80g of purified water
•Direct-reading balance (Weighing capacity: Min. 200g, Accuracy: ±0.005g)
•100mL beaker (Made of glass or polyethylene)
•Spoon (Made of plastic)
②Procedure
•Place the beaker on the balance and set the indication of the balance to 0.00g.
•Put 20.00g of sucrose in the beaker.
•Add 80.00g of purified water to the sucrose so that the total weight will be 100.00g.
•Take down the beaker from the balance and stir the mixture well until all the sucrose is dissolved.
③Precautions for making the standard sucrose solution
•The unit of Brix of the refractometer is weight/weight%.
•The sucrose solution should be made so that its total weight will be 100g (If the total weight is
less than 100g, the relative error is increased).
•Keep the prepared sucrose solution in a sealed container.
•Purchase sucrose from a reagent shop, drug store or pharmacy.
