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Evolve™ is a trademark of Delta Precision Ltd.

HydroPure™ is a trademark of Contec.

Santoprene™ is a trademark of ExxonMobil.

PuraBead

- this product or portions thereof is manufactured and sold under license from GE

Healthcare under US patents 6841097 and 7341202 and any corresponding patents for life

sciences research and the purification/separation of naturally occurring or recombinant
plasma derived proteins.

All other trademarks, trade names, trade dress, product names and logos appearing on this
document are the property of Astrea Bioseparations Ltd.

Содержание Evolve AB070505

Страница 1: ...Technical User Guide Evolve Process Columns 70 100 140mm diameter columns...

Страница 2: ...PC Evolve Columns Operating Manual v2 1...

Страница 3: ...rization 12 Handling 12 Chemical compatibility 12 3 Column Installation and Operation 13 Column Installation 13 1 Installation of bed supports 13 2 Installation of Fixed Bed Support 13 3 Levelling the...

Страница 4: ...t 30 Connection kit 30 Spares Kit 31 8 Spare Parts Kits Accessories 32 Column Refresh Kit 32 Wetted Flow path Kit 34 Bed Support Kit 34 Elastomer Seal Kit 35 Adjuster Cell Kit 35 Tube Replacement Kits...

Страница 5: ...e column with part identification 19 Figure 9 Adjuster Assembly for EvolveTM 100 Process Column 22 Figure 10 Fixed Assembly for EvolveTM 100 Process Column 23 Figure 11 Diagram showing the location of...

Страница 6: ...as the following advantages a Ensures the maximum area is available to flow promoting good chromatographic performance and clean ability b Eliminates use of fixings to secure the bed support to the fl...

Страница 7: ...4 58 4 58 Operating temperature 2 to 30 C Bed support size 8 m Weights and Dimensions Column 70 100 140 Product Code AB070505 AB100505 AB200505 Adjuster Weight kg 4 5 7 5 Total weight empty kg 11 5 1...

Страница 8: ...mn outlet shows the contribution made by the column hardware The tests were performed at ambient temperature 15 20 C using RO water Figure 1 Pressure Drop Evolve 70 mm Process Column Figure 2 Pressure...

Страница 9: ...PC Evolve Columns Operating Manual v2 8 Figure 3 Pressure Drop Evolve 140 mm Process Column...

Страница 10: ...Flow tube seal fixed and adjuster Santoprene Adjuster bed support seal Santoprene Fixed flow cell Polypropylene black Adjuster flow cell Polypropylene black Anti jet disc 20 glass filled polypropylene...

Страница 11: ...wise Concentrations refer to w w All data is referenced to room temperature 15 25 C Chemical Wetted Components Non wetted Stainless Steel 316L 1 2 dichloroethane Acetic acid 25 Acetone 5 Acetonitrile...

Страница 12: ...ketone MEK Methylene chloride Nitric acid 10 Nitric acid 70 Peracetic acid 300 ppm Phosphoric acid 55 Potassium hydroxide 2 0 M Iso propanol 20 Iso propanol 40 n propanol 20 Sodium acetate Sodium bica...

Страница 13: ...tances that will be used in the column will remain in liquid form during the entire operation at the maximum stated operating temperature In addition The maximum pressure is less than 6 bar The produc...

Страница 14: ...eling the column Setting the bed height 1 Installation of bed supports The Evolve Process Column features polypropylene woven mesh bed supports integrated with a Santoprene seal These do not require p...

Страница 15: ...cated in the foot ring of the column Place the spirit level on the tube flange and adjust the levelling studs to ensure column is level before proceeding There is approximately 30 mm free vertical mov...

Страница 16: ...support 5 Setting the Bed Height Reinstate the adjuster unit in the acrylic tube using the location pins and ensure the adjuster seal is de energized Replace the flange fixing bolts underneath the tub...

Страница 17: ...to the correct torque setting See table below 2 Fill the column with water for injection WFI or high quality water and position the adjuster at the operating bed height 3 Ensure the adjuster seal is...

Страница 18: ...low cell towards the slurry by turning the height adjust handles but do not enter the slurry mixture 13 The slurry will start to settle when a liquid interface is seen gently lower the adjuster into t...

Страница 19: ...ed height has been reached turn the seal actuation knob to apply maximum seal compression 21 After the final compression step flow in the upwards direction to remove any residual air at the top of the...

Страница 20: ...PC Evolve Columns Operating Manual v2 19 Figure 8 Schematic of the column with part identification...

Страница 21: ...ed that the packed column is stored in 20 ethanol 80 0 1 M NaCl solution at 2 30 C For in process storage the packed column may be stored in any compatible solvent refer to the Chemical Compatibility...

Страница 22: ...are not to damage the edge of the flow cells 5 When unpacking columns be careful not to scratch the inside of the acrylic column tubes 6 Be careful not to damage stainless steel surfaces by bumping kn...

Страница 23: ...clamp adapter and retain flow tube seal 6 Remove the seal adjust nut and spacer and slide the flow tube and seal adjust tube out of the threaded rod 7 Remove the flow cell locking clip and retain Slid...

Страница 24: ...a clean work surface 3 Locate the flats on the process flow tube and using the correct tool remove the flow tube Retain and inspect the flow tube seal 4 To remove the mesh seal assembly from the flow...

Страница 25: ...flow tube using correct tools 2 Install the bed support seal assembly on to the flow cell by carefully locating the bed support seal onto the flow cell The bed support should be fitted with the smooth...

Страница 26: ...ng Nm Torque Setting Ft lbf 140 mm 20 14 75 100 mm 20 14 75 70 mm 20 14 75 5 Fit the pipework elbow and ensure all connections are secure Note do not over tighten Adjuster Assembly 1 Install the adjus...

Страница 27: ...ainless steel central tube and replace the flow cell locking clip Figure 15 Photograph showing the final location of the adjuster bed support 5 Locate the flow tube stainless steel unit inside the thr...

Страница 28: ...sanitary clamp adapter to ensure component is correctly tightened 9 Lift the adjuster unit into the acrylic column tube Note alignment pins are located in the top flange to assist orientation CAUTION...

Страница 29: ...under cold room conditions or with cold process fluids retighten all bolts If possible empty the column and removed the fixed cell assembly Remove the fixed bed support with integral seal and inspect...

Страница 30: ...ecipitate it is possible that the bed supports have become blocked This can also occur if the chromatographic media contains fines If this is suspected replace the bed supports and repeat the pressure...

Страница 31: ...open ended 1 X X X Fixed end tool Small 1 X X X Torque wrench 3 8 drive 1 X X X Spirit level 1 X X X Seal tool Micro S driver slotted 1 2 mm 1 X X X Connection kit DESCRIPTION QTY PRODUCT NUMBER AA07...

Страница 32: ...support assembly 100 Fixed 1 X Bed support assembly 100 Adjuster 1 X Bed support assembly 140 Fixed 1 X Bed support assembly 140 Adjuster 1 X Adjuster seal Evolve 70 mm 1 X Adjuster seal Evolve 100 mm...

Страница 33: ...X Flow Tube Seal 7 0 mm Bore 3 X Adjuster seal 70 mm Column 1 X Adjuster seal 100 mm Column 1 X Adjuster seal 140 mm Column 1 X Pipe spool Elbow 70 mm Column 1 X Pipe spool Elbow 100 mm Column 1 X Pi...

Страница 34: ...Fixed Cell 140 mm 1 X Acrylic Tube 70 X 530 mm 1 X Acrylic Tube 100 X 530 mm 1 X Acrylic Tube 140 X 530 mm 1 X Buffer Ring 70 mm 2 X Buffer Ring 100 mm 2 X Buffer Ring 140 mm 2 X Scale Label 70 mm 1...

Страница 35: ...w 70 mm Column 1 X Pipespool Elbow 100 mm Column 1 X Pipespool Elbow 140 mm Column 1 X Flow Tube Seal Ladish 3 83 mm Bore 3 X X Flow Tube Seal Ladish 7 0 mm Bore 3 X Bed Support Assembly 70 Fixed 1 X...

Страница 36: ...DESCRIPTION QTY PRODUCT NUMBER AA07ACK AA10ACK AA14ACK Adjuster Cell 1 X Adjuster Cell 1 X Adjuster Cell 1 X Flow Cell Locking Clip 70 100 mm 1 X X Flow Cell Locking Clip 140 mm 1 X Anti jet 1 X X X T...

Страница 37: ...eter x 1 0 m 2 3 mm Tee 2 Click Clamp 3 4 Ladish 10 Flow Tube Seal Ladish 3 83 mm Bore 10 Blanking Cap 2 Fluid Connection Kit 140 200 250 mm diameter columns DESCRIPTION QTY Product Number 2 Way Sanit...

Страница 38: ...DESCRIPTION QTY Product Number Flexible Hose 6 mm Diameter x 2 0 m 2 AAFHK2 Flow Tube Seal Ladish 7 0 mm Bore 2 Click Clamp 3 4 Ladish 2 Torque Wrench Kit 70 100 140 mm diameter columns DESCRIPTION Q...

Страница 39: ...ctivity is measured and plotted The plot obtained is then analysed to calculate the following terms of efficiency Chromatographic efficiency is expressed as HETP height equivalent to theoretical plate...

Страница 40: ...e Pd is the mean particle diameter Where the initial sample volume and the values of a and b are all expressed in the same volume units Note comparison of results should be undertaken with caution Dif...

Страница 41: ...cense from GE Healthcare under US patents 6841097 and 7341202 and any corresponding patents for life sciences research and the purification separation of naturally occurring or recombinant plasma deri...

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