12
Perform an experiment
Applied Biosystems ViiA
™
7 Real-Time PCR System Quick Reference Guide
5
Seal the array card
(continued)
d.
Seal the array card by running the carriage slowly over it, in
one direction only.
e.
Remove the sealed array card from the fixture and trim the
fill reservoirs from the array card assembly using scissors. Trim the
foil array card so that the edge is even with the plastic carrier.
Completely remove the fill reservoirs from the array card so that the
edge is free of residual plastic. The plastic from the fill reservoirs
that extends beyond the edge of the card can prevent the card from
seating properly on the sample block and affect amplification.
IMPORTANT!
Keep the filled array card out of the light. The fluorescent dye in the array card is
photosensitive. Prolonged exposure to light can diminish the fluorescence of the dye.
Start and monitor the experiment
1
Load the plate or array
card
a.
Touch
on the instrument touchscreen to eject the plate adapter.
b.
Place the plate or array card on the plate adapter. Ensure that the plate or array card is properly
aligned in the holder:
• Load plates and array cards with the A1 position aligned with the A1 position of the tray.
• Load plates and array cards with the barcode facing the front of the instrument.
IMPORTANT!
Plates should loaded and unloaded by trained operators that have been warned
of the moving parts hazard.
c.
Touch
to load the plate.
Correct
Incorrect
