Applied Biosystems GeneAmp PCR System 9700, Руководство пользователя

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6-4 Converting Hold Times
Up Ramp Example
In this example, the temperature was increased by 17 °C. This value was rounded to
15 °C. According to Table 6-1, subtract 45 seconds from the hold time on the DNA
Thermal Cycler or the DNA Thermal Cycler 480, resulting in a new hold time of
35 seconds (see the figure below).
Note If methods developed on the GeneAmp PCR System 9700 will be used on the DNA
Thermal Cycler or the DNA Thermal Cycler 480, you can also use Table 6-1 to convert the hold
times. Add the indicated times instead of subtracting them.
Setting Down Ramps
The following table lists the process of setting the down ramp temperature.
80 sec.
Hold
Time
72
55
Temp.
( ˚ C)
∆
temp=17
˚
(round to 15
˚
)
DNA Thermal Cycler
35 sec.
Hold
Time
72
55
Temp.
( ˚ C)
GeneAmp PCR System 9700
Step Action
1 Determine the change in temperature required to reach the next target temperature
and round this value off to the closest value found in Table 6-2.
2 Based on the starting temperature of the ramp, determine the number of seconds to
subtract from the DNA Thermal Cycler or DNA Thermal Cycler 480 hold time to
arrive at the new hold time.
Note The typical hold time is 10 to 15 seconds for annealing.