Antec Scientific DECADE II 171, Руководство пользователя

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70 DECADE II user manual, ed. 11
Fig. 43. Chromatogram of the analyte plug obtained in the DC mode.
Scanning takes place on top of the broad peak between 0.5 and 2.5 min after
injection in FIA mode.
5. The sampling frequency of the integrator is set at 1 Hz. This is the
same frequency as the voltage steps during the scan. If a higher
sampling frequency is chosen a typical stepwise pattern may appear.
6.
In the ‘SCAN SETUP’ screen an upper and a lower potential is
chosen. The cycle is set at half. The range is set at 5 µA. A scan
speed of 10 mV/s is selected.
7. The analyte is injected and the scan is started by pressing the
‘START’ in the ‘SCAN STAT’ screen of the DECADE II at the time
the analyte plug enters the flow cell (see 4).
8. A background scan is obtained by scanning the HPLC buffer.
For reliable results it is recommended to repeat each scan three times.
Using scan mode in ALEXYS data system
The ALEXYS data system offers a convenient interface to construct a
scanning voltammogram. Open the DECADE II device driver and click on
‘Scan Shell’. This opens the actual scan window and half or full scans can be
acquired. Continuous scanning is done by recording multiple full scans after
each other.