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Chapter 5: Optimizing seal quality
PlateLoc User Guide
Optimization test guidelines
About this topic
This topic presents the guidelines to consider when planning seal-
quality optimization tests.
Test guidelines
When you plan optimization tests, consider the following:
❑
Always run an optimization test for each type of plate material and
plate design. Plates made from the same material but with different
designs can require different sealing parameters.
❑
Always use spare plates in the optimization tests.
❑
Use a new plate for each seal cycle. The characteristics of a plate
can change through repeated seal cycles, therefore changing the
seal results.
❑
Consider the plate content and fluid level in the plate.
❑
Consider the downstream application. For example, plates used in a
thermal cycler will require different sealing parameters than plates
placed in the freezer.
❑
Consider the downstream uses of the plate. For example, plates that
will be sealed one time and discarded can have a more permanent
seal than a plate that will be reused and resealed repeatedly before
being discarded.
❑
Start an optimization test with a low-temperature and short-duration
setting. Adjust the parameters in small increments.
❑
Check the quality of the seal after each seal cycle to determine
which sealing parameters to adjust and whether you need to use an
insert.
❑
Record the optimal sealing parameters.
For seal selection, insert selection, and other optimization test
guidelines, see the
PlateLoc Thermal Plate Sealer Seal Selection Guide
.
You can locate the guide on the Velocity11 website at
www.velocity11.com.
Related topics
For more information about...
See...
Reasons for running optimization
tests
“Reasons for running optimization
tests” on page 78
Plate requirements
“Plate requirements” on page 9
Seal types
Insert types
Adjusting sealing parameters
Содержание PlateLoc
Страница 1: ...Agilent Technologies PlateLoc Thermal Microplate Sealer User Guide...
Страница 4: ......
Страница 54: ...Chapter 2 Unpacking and installation PlateLoc User Guide 40...
Страница 78: ...Chapter 3 Getting started PlateLoc User Guide 64...
Страница 90: ...Chapter 4 Sealing plates PlateLoc User Guide 76...
Страница 129: ...115 Appendix A Integrating the PlateLoc into third party systems PlateLoc User Guide Returns None...
Страница 130: ...Appendix A Integrating the PlateLoc into third party systems PlateLoc User Guide 116...
Страница 138: ...Appendix C Glossary PlateLoc User Guide 124...
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Страница 143: ......
Страница 144: ...Agilent Technologies User Guide G5402 90001...