Agilent DNA 1000 Kit Quick Start Guide
*G2938-90015*
*G2938-90015*
G2938-90015 Rev. D
Edition 12/2017
For Research Use Only
Not for use in diagnostic procedures
Printed in Germany
© Agilent Technologies, Inc. 2000-2016, 2017
Agilent Technologies
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76337 Waldbronn, Germany
Preparing the Gel-Dye Mix
1
Allow DNA dye concentrate (blue ) and DNA gel matrix (red ) to equilibrate to room temperature for
30 min.
2
Add 25 μL of DNA dye concentrate (blue ) to a DNA gel matrix vial (red ).
3
Vortex solution well and spin down. Transfer to spin filter.
4
Centrifuge at 2240 g ± 20 % for 15 min. Protect solution from light. Store at 4 °C.
Use prepared gel-dye mix within 6 weeks of preparation.
Loading the Gel-Dye Mix
1
Allow the gel-dye mix to equilibrate to room temperature for 30 min before use.
2
Put a new DNA chip on the chip priming station.
3
Pipette 9 μL of gel-dye mix in the well marked
.
4
Make sure that the plunger is positioned at 1 mL and then close the chip priming station.
5
Press plunger until it is held by the clip.
6
Wait for exactly 60 s then release clip.
7
Wait for 5 s, then slowly pull back the plunger to the 1 mL position.
8
Open the chip priming station and pipette 9 μL of gel-dye mix in the wells marked
.
Loading the Marker
1
Pipette 5 μL of marker (green ) in all sample and ladder wells. Do not leave any wells
empty.
Loading the Ladder and Samples
Technical Support
Please visit our support web page
http://www.agilent.com/genomics/contactus
to find information on your
local Contact Center.
Further Information
Visit the 2100 Bioanalyzer site at
http://www.agilent.com/genomics/bioanalyzer
. You can find useful
information, support and current developments about the products and the technology.
1
Pipette 1 µL of DNA ladder (yellow ) in the well marked
.
2
In each of the 12 sample wells pipette 1 µL of sample (used wells) or 1 µL of de-ionized
water (unused wells).
3
Put the chip horizontally in the adapter and vortex for 1 min at the indicated setting
(2400 rpm).
4
Run the chip in the Agilent 2100 Bioanalyzer instrument within 5 min.
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