2
Agilent DNA 1000 Kit Quick Start Guide
Storage Conditions
•
Keep all reagents and reagent mixes refrigerated at 4 °C when not in use to avoid poor results caused by
reagent decomposition.
•
Protect dye and dye mixtures from light. Remove light covers only when pipetting. Dye decomposes when
exposed to light.
Equipment Supplied with the Agilent 2100 Bioanalyzer
•
Chip priming station (reorder number 5065-4401)
•
IKA vortex mixer
Additional Material Required (Not Supplied)
•
Pipettes (10 μl, 100 μl and 1000 μl) with compatible tips
•
0.5 ml microcentrifuge tubes for sample preparation
•
Microcentrifuge
Sample Preparation
•
PCR samples: For accurate determination of DNA concentration, the total DNA in sample must be
between 0.1–50 ng/μl. If concentration of your particular PCR reaction is excessively high, dilute to
0.1–50 ng/μl.
•
Restriction digests: Final concentration of DNA should not exceed 50 ng/μl. Add EDTA and/or heat
inactivate the restriction enzyme according to the manufacturer instructions. Restriction endonucleases
in combination with non-chelated metal ions may degrade internal DNA markers used in assay kit.
Table 2
DNA 1000 Kit Specifications
Physical Specifications
Analytical Specifications
Type
Specification
Type
Agilent DNA 1000 Assay
Analysis time
35 min
Sizing range
25 – 1000 bp
Number of
samples
12 samples/chip
Sizing resolution
25 – 100 bp: 5 bp
100 – 500 bp: 5 %
500 – 1000 bp: 10 %
Sample volume
1 µL
Sizing accuracy
± 10 %
Kit stability
4 months (see box for storage
temperatures)
Sizing reproducability
5 % CV
Kit size
25 chips
12 samples/chip
= 300 samples/kit
Quantitation accuracy
1
1
Determined using the respective DNA ladder as sample
20 %
Quantitation reproducibility
1
25 – 500 bp: 15 % CV
500 – 1000 bp: 5 % CV
Quantitative range
1
0.5 – 50 ng/µL
Maximum salt concentration in
sample
250 mM for KCl,
15 mM for MgCl
2
,
250 mM NaCl
