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Tipps für optimale Chromatografieergebnisse

• Optimieren Sie Ihr Instrument, indem Sie die

Längen der Kapillaren zwischen den Komponenten

minimieren, um zusätzliches Säulenvolu-men und

Bandenverbreiterung zu reduzieren. Verwenden Sie rote

Kapillaren mit einem Innendurchmesser von 0.12 mm für

Fast-LC-/ Hocheffizienzsäulen. Weitere Informationen zu

Kapillaroptionen erhalten Sie unter

agilent.com/chem/lccapillaries

• Stellen Sie sicher, dass die Datenerfassungsrate für

Ihre Säule optimiert ist. Verwenden Sie eine höhere

Datenerfassungsrate für Fast-LC-Säulen (Poroshell 120,

RRHT, RRHD).

• Filtrieren Sie Ihre Probe oder führen Sie eine für Ihre Probe

geeignete andere Probenvorbereitung durch. Weitere

Informationen finden Sie unter

agilent.com/chem/sampleprep

• Verwenden Sie für eine optimale Leistung

Agilent zertifizierte Lampen in Ihren

Flüssigkeitschromatografiegeräten.

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Содержание and C8

Страница 1: ...nbenutzerhandbuch f r Agilent Umkehrphasens ulen Manuale utenti per colonne a fase inversa Agilent Gu a de usuario de columna para columnas de fase reversa Agilent Agilent ZORBAX reversed phase phase...

Страница 2: ...odified from a standard system to minimize system dead volume so it may vary from the system used in your lab This allows a better evaluation of the column and assures a more consistent product A prop...

Страница 3: ...e Recommended Fitting Part Numbers Up to 400 bar InfinityLab Quick Turn Fitting finger tight Fitting 5067 5966 Up to 800 bar InfinityLab Quick Turn Fitting with Mounting tool Fitting 5067 5966 Mountin...

Страница 4: ...ng Important safety considerations All points of connection in liquid chromatographic systems are potential sources of leaks Users should be aware of the toxicity or flammability of their mobile phase...

Страница 5: ...se of a guard column is recommended to protect your column and increase its lifetime Columns should not be maintained at elevated pH or elevated temperature when not in use Avoid use of this column ou...

Страница 6: ...d C18 ZORBAX Rx C8 ZORBAX SB Aq ZORBAX SB Phenyl SB C3 Poroshell 300 300SB C3 pH 1 0 to 8 0 90 C StableBond C18 and Poroshell 120 SB C18 80 C Rx C8 and SB Aq SB C3 and SB Phenyl 70 C for pH 5 0 40 C f...

Страница 7: ...BAX Rapid Resolution High Definition RRHD 1 8 m 1200 bar 17 000 psi All ZORBAX Pursuit and Polaris columns not noted as RRHT or RRHD and HC C18 2 TC C18 2 3 0 m 3 5 m 5 m 400 bar 6000 psi 200 bar 3000...

Страница 8: ...X Bonus RP Lower concentration of organic mobile phase modifier is required for compound elution compared to traditional long chain alkyl stationary phases Large molecule columns for example Poroshell...

Страница 9: ...d Polaris columns start with a stronger less polar solvent 1 Disconnect column from detector and run wash solvents into a beaker 2 Start with your mobile phase without buffer salts water organic Run a...

Страница 10: ...pure solvent Before storing end fittings should be tightly capped with end plugs to prevent packing from drying out Columns may be safely stored for short periods in most mobile phases To protect equi...

Страница 11: ...Fast LC high efficiency columns Learn about capillary options at agilent com chem lccapillaries Ensure the data collection rate is optimized for your column Use a higher collection rate for Fast LC c...

Страница 12: ...ZORBAX Poroshell Pursuit Polaris agilent com chem columnchoices Agilent QC QC LC QC...

Страница 13: ...Up to 800 bar InfinityLab Quick Turn Fitting with Mounting tool Fitting 5067 5966 Mounting Tool 5043 0915 Up to 1300 bar InfinityLab Quick Connect Fitting Fitting 5067 5965 For more information and pa...

Страница 14: ...10 20 C8 CN...

Страница 15: ...QC Agilent pH pH pH Poroshell 120 ZORBAX RRHT RRHD Fast Guard agilent com chem fastguards...

Страница 16: ...300 300SB C3 ZORBAX Eclipse Plus C18 C8 Eclipse XDB C8 XDB C18 XDB Phenyl Poroshell 120 EC C18 EC C8 ZORBAX Bonus RP HC C18 2 and TC C18 2 pH 2 0 9 0 60 C ZORBAX Extend C18 pH 2 0 11 5 60 C pH 40 C P...

Страница 17: ...si ZORBAX 3 5 m 400 bar 6000 psi ZORBAX RRHT 1 8 m 600 bar 9000 psi ZORBAX RRHD 1 8 m 1200 bar 17 000 psi ZORBAX Pursuit Polaris RRHT RRHD HC C18 2 TC C18 2 3 0 m 3 5 m 5 m 400 bar 6000 psi 9 4 5 100...

Страница 18: ...2 7 m 2 m ZORBAX Fast Guard agilent com chem fastguards Poroshell 120 1 8 m ZORBAX Pursuit Polaris 1 2 10 20 3 100 4 5 75 25 6 100 100 100 1 8 m...

Страница 19: ...50 50 20 30 20 30 60 40 ACN H20 60 40 ACN 0 02 M LC 0 12 agilent com chem lccapillaries LC Poroshell 120 RRHT RRHD agilent com chem sampleprep LC Agilent...

Страница 20: ...olumes morts dans le syst me de sorte qu il peut tre diff rent du syst me utilis dans votre laboratoire Cela permet une meilleure valuation de la colonne et garantit une meilleure conformit du produit...

Страница 21: ...tilis es aux pressions UHPLC Maximum System Pressure Recommended Fitting Part Numbers Up to 400 bar InfinityLab Quick Turn Fitting finger tight Fitting 5067 5966 Up to 800 bar InfinityLab Quick Turn F...

Страница 22: ...it Tous les points de connexion dans les syst mes de chromatographie liquide sont des sources potentielles de fuite Les utilisateurs doivent tre inform s de la toxicit et de l inflammabilit de leurs p...

Страница 23: ...ganiques communs L utilisation d une colonne de garde est recommand e pour prot ger la colonne et augmenter sa dur e de vie Les colonnes ne doivent pas tre maintenues un pH lev ni une temp rature lev...

Страница 24: ...Phenyl 70 C pour un pH 5 0 40 C pour un pH de 5 0 8 0 Poroshell 300 et 300SB C3 ZORBAX Eclipse Plus C18 et C8 Eclipse XDB C8 XDB C18 XDB Phenyl Poroshell 120 EC C18 et EC C8 ZORBAX Bonus RP HC C18 2...

Страница 25: ...de pression Poroshell 120 1 9 m 2 7 m 4 m 1300 bar 19 500 psi 600 bar 9000 psi Poroshell 300 5 m 400 bar 6000 psi ZORBAX R solution Rapide 3 5 m 400 bar 6000 psi ZORBAX R solution Rapide Haut D bit RR...

Страница 26: ...solvant organique inf rieure est requise pour l lution du compos par rapport aux phases stationnaires longue cha ne alkyle traditionnelles Colonnes pour les biomol cules par ex les colonnes Poroshell...

Страница 27: ...es Pursuit et Polaris d marrez avec un solvant plus fort moins polaire 1 D branchez la colonne du d tecteur La phase mobile sortant de la colonne sera r cup r e dans un b cher 2 D marrez avec la phase...

Страница 28: ...t le stockage veillez bien fermer les extr mit s de la colonne afin d viter le s chage Les colonnes peuvent tre stock es en toute s curit pour de courtes p riodes dans la plupart des phases mobiles Af...

Страница 29: ...rents tubes capillaires disponibles consultez le site agilent com chem lccapillaries V rifiez que la vitesse d acquisition des donn es est optimis e pour votre colonne Utilisez une vitesse d acquisit...

Страница 30: ...minimieren Es kann also von dem in Ihrem Labor verwendeten System abweichen Die S ule kann so besser evaluiert und eine konsistente Produktqualit t sichergestellt werden Ein ordnungsgem konfigurierte...

Страница 31: ...cken verwendet werden Maximum System Pressure Recommended Fitting Part Numbers Up to 400 bar InfinityLab Quick Turn Fitting finger tight Fitting 5067 5966 Up to 800 bar InfinityLab Quick Turn Fitting...

Страница 32: ...tige Sicherheitshinweise Alle Anschlu punkte in LC Systemen sind potentielle Quellen von Lecks Benutzer sollten die Toxizit t bzw Brennbarkeit der mobilen Phase kennen Die S ule solte nur in gut bel f...

Страница 33: ...ie Verwendung einer Vors ule wird zum Schutz der S ule und zur Verl ngerung ihrer Lebenszeit empfohlen Zur Zeit nicht benutzte S ulen sollten keine erh hten pH Werte oder Temperaturen ausgesetzt werde...

Страница 34: ...ten 5 0 40 C f r pH Werte 5 0 bis 8 0 Poroshell 300 und 300SB C3 ZORBAX Eclipse Plus C18 und C8 Eclipse XDB C8 XDB C18 XDB Phenyl Poroshell 120 EC C18 und EC C8 ZORBAX Bonus RP HC C18 2 and TC C18 2 p...

Страница 35: ...en S ulentyp Partikelgr e Druckgrenzwert Poroshell 120 1 9 m 2 7 m 4 m 1300 bar 19 500 psi 600 bar 9000 psi Poroshell 300 5 m 400 bar 6000 psi ZORBAX Rapid Resolution 3 5 m 400 bar 6000 psi ZORBAX Rap...

Страница 36: ...ettigen station ren Alkylphasen ist f r die Elution von chemischen Verbindungen eine niedrigere Konzentration des organischen Modifikators der mobilen Phase erforderlich S ulen f r gro e Molek le z B...

Страница 37: ...s weniger polares L sungsmittel verwendet werden 1 Trennen Sie die S ule vom Detektor und f llen Sie die L sungsmittel in ein Becherglas 2 Beginnen Sie mit der mobilen Phase ohne Puffersalze Wasser or...

Страница 38: ...t wird Vor der Lagerung sollten die Endverschraubungen fest mit Endverschl ssen verschlos sen werden um ein Austrocknen der Packung zu verhindern Kurzfristig k nnen S ulen in den meisten mobilen Phase...

Страница 39: ...onen zu Kapillaroptionen erhalten Sie unter agilent com chem lccapillaries Stellen Sie sicher dass die Datenerfassungsrate f r Ihre S ule optimiert ist Verwenden Sie eine h here Datenerfassungsrate f...

Страница 40: ...andard riducendo cos al minino il volume morto del sistema possibile assistere a delle variazioni rispetto al sistema utilizzato in laboratorio In questo modo si pu meglio valutare la colonna e garant...

Страница 41: ...ssioni dell UHPLC Maximum System Pressure Recommended Fitting Part Numbers Up to 400 bar InfinityLab Quick Turn Fitting finger tight Fitting 5067 5966 Up to 800 bar InfinityLab Quick Turn Fitting with...

Страница 42: ...e che prima di essere utilizzata la colonna sia stata equilibrata correttamente per garantire riproducibilit e prevenire variazioni nel tempo di ritenzione Importanti considerazioni sulla sicurezza Tu...

Страница 43: ...e la fase mobile attraversi la colonna per non causare la formazione di un precipitato Le colonne a fase inversa Agilent sono compatibili con l acqua e con tutti i comuni solventi organici Si consigli...

Страница 44: ...enyl 70 C a pH 5 0 40 C a pH da 5 0 a 8 0 Poroshell 300 e 300SB C3 ZORBAX Eclipse Plus C18 e C8 Eclipse XDB C8 XDB C18 XDB Phenyl Poroshell 120 EC C18 and EC C8 ZORBAX Bonus RP HC C18 2 e TC C18 2 da...

Страница 45: ...ell 120 1 9 m 2 7 m 4 m 1300 bar 19 500 psi 600 bar 9000 psi Poroshell 300 5 m 400 bar 6000 psi ZORBAX rapida risoluzione 3 5 m 400 bar 6000 psi ZORBAX rapida risoluzione elevato rendimento RRHT 1 8 m...

Страница 46: ...ZORBAX Bonus RP Rispetto alle fasi stazionarie con lunghe catene alchiliche per l eluizione del composto necessario utilizzare una concentrazione pi bassa del modificatore della fase mobile Colonne pe...

Страница 47: ...ne Pursuit e Polaris iniziare utilizzando un solvente pi forte o meno polare 1 Scollegare la colonna dal rivelatore e versare i solventi per il lavaggio in un bicchiere 2 Iniziare con la fase mobile s...

Страница 48: ...uro Per evitare che il riempimento si asciughi tappare bene le estremit prima dello stoccaggio Le colonne possono restare stoccate nelle maggior parte della fasi mobili per brevi periodi Per conservar...

Страница 49: ...ioni sulle soluzione capillari consultare il sito agilent com chem lccapillaries Controllare che la velocit di raccolta dei dati sia ottimale per la colonna Utilizzare una velocit pi alta in caso di c...

Страница 50: ...o optimizado para minimizar el volumen muerto del sistema por lo que puede variar del sistema que utiliza en su laboratorio Esto permite una mejor evaluaci n de la columna y garantiza un producto m s...

Страница 51: ...as a presiones UHPLC Maximum System Pressure Recommended Fitting Part Numbers Up to 400 bar InfinityLab Quick Turn Fitting finger tight Fitting 5067 5966 Up to 800 bar InfinityLab Quick Turn Fitting w...

Страница 52: ...raciones de seguridad Todos los puntos de conexi n en los sistemas cromatogr ficos l quidos son potenciales fuentes de fugas Los usuarios deben ser conscientes de la toxicidad e inflamabilidad de sus...

Страница 53: ...n todos los disolventes org nicos comunes Se recomienda el uso de una salvacolumna para proteger la columna y aumentar su vida til Las columnas no deben mantenerse a elevado pH o elevada temperatura c...

Страница 54: ...enyl 70 C para pH 5 0 40 C para pH 5 0 a 8 0 Poroshell 300 and 300SB C3 ZORBAX Eclipse Plus C18 y C8 Eclipse XDB C8 XDB C18 XDB Phenyl Poroshell 120 EC C18 and EC C8 ZORBAX Bonus RP HC C18 2 y TC C18...

Страница 55: ...e presi n Poroshell 120 1 9 m 2 7 m 4 m 1300 bar 19 500 psi 600 bar 9000 psi Poroshell 300 5 m 400 bar 6000 psi ZORBAX de resoluci n r pida 3 5 m 400 bar 6000 psi ZORBAX de resoluci n r pida alto rend...

Страница 56: ...ntraci n de como modificador el fase m vil org nico para eluci n del compuesto en comparaci n con las fases estacionarias alqu licas de cadena larga tradicionales Columnas de mol culas grandes por eje...

Страница 57: ...lumnas Poroshell 120 y columnas ZORBAX con part culas 1 8 m y todas las columnas Pursuit y Polaris comience con un disolvente m s fuerte menos polar 1 Desconecte la columna del detector y eche los dis...

Страница 58: ...guido de 20 a 30 vol menes de columna del disolvente puro Antes del almacenami ento se deben tapar bien las conexiones finales con tapones para evitar que el relleno se reseque Las columnas se pueden...

Страница 59: ...tenga informaci n sobre las opciones de capilares en agilent com chem lccapillaries Aseg rese de que la velocidad de adquisici n de datos para la columna est optimizada Utilice una velocidad de adquis...

Страница 60: ...ZORBAX Poroshell Pursuit Polaris agilent com chem columnchoices Agilent QC QC LC QC LC...

Страница 61: ...r InfinityLab Quick Turn Fitting with Mounting tool Fitting 5067 5966 Mounting Tool 5043 0915 Up to 1300 bar InfinityLab Quick Connect Fitting Fitting 5067 5965 For more information and part numbers p...

Страница 62: ...10 20 C8 CN QC...

Страница 63: ...Agilent pH pH pH Poroshell 120 ZORBAX RRHT 1 8 um RRHD Fast Guard agilent com chem fastguards...

Страница 64: ...pH 5 0 40 C pH 5 0 8 0 Poroshell 300 300SB C3 ZORBAX Eclipse Plus C18 C8 Eclipse XDB C8 XDB C18 XDB Phenyl Poroshell 120 EC C18 EC C8 ZORBAX Bonus RP HC C18 2 TC C18 2 pH 2 0 9 0 60 C ZORBAX Extend C...

Страница 65: ...m 400 bar 6000 psi ZORBAX Rapid Resolution 3 5 m 400 bar 6000 psi ZORBAX Rapid Resolution High Throughput RRHT 1 8 m 600 bar 9000 psi ZORBAX Rapid Resolution High Definition RRHD 1 8 m 1200 bar 17 00...

Страница 66: ...5 100 ZORBAX Eclipse PAH 30 40 100 15 20 C ZORBAX Bonus RP Poroshell 300 ZORBAX 300SB DNA pH pH TRIS 40 C...

Страница 67: ...agilent com chem fastguards 1 2 10 20 3 100 4 5 4 1 8 m...

Страница 68: ...20 30 50 50 20 30 60 40 ACN 0 02 M 60 40 ACN H20...

Страница 69: ...0 12 mm agilent com chem lccapillaries Poroshell 120 RRHT RRHD agilent com chem sampleprep LC Agilent...

Страница 70: ...ZORBAX Pursuit Polaris agilent com chem columnchoices Agilent...

Страница 71: ...800 bar InfinityLab Quick Turn Fitting with Mounting tool Fitting 5067 5966 Mounting Tool 5043 0915 Up to 1300 bar InfinityLab Quick Connect Fitting Fitting 5067 5965 For more information and part nu...

Страница 72: ...10 20 C8 CN...

Страница 73: ...Agilent pH pH pH Poroshell 120 ZORBAX RRHT RRHD 1 8 Fast Guard UHPLC agilent com chem fastguards...

Страница 74: ...0 8 0 Poroshell 300 300SB C3 ZORBAX Eclipse Plus C18 C8 Eclipse XDB C8 XDB C18 XDB Phenyl Poroshell 120 EC C18 and EC C8 ZORBAX Bonus RP HC C18 2 TC C18 2 pH 2 0 9 0 60 C ZORBAX Extend C18 pH 2 0 11...

Страница 75: ...9000 psi Poroshell 300 5 400 6000 psi ZORBAX Rapid Resolution 3 5 400 6000 psi ZORBAX Rapid Resolution High Throughput RRHT 1 8 600 9000 psi ZORBAX Rapid Resolution High Definition RRHD 1 8 1200 17 00...

Страница 76: ...5 100 ZORBAX Eclipse PAH 30 40 100 15 20 C ZORBAX Bonus RP Poroshell 300 ZORBAX 300SB pH TRIS pH 40 C...

Страница 77: ...2 7 2 ZORBAX Fast Guard agilent com chem fastguards Poroshell 120 ZORBAX 1 8 Pursuit Polaris 1 2 10 20 3 100 4 5 75 25...

Страница 78: ...6 100 100 100 1 8 50 50 20 30 20 30 H2O 60 40 60 40 0 02...

Страница 79: ...0 12 agilent com chem lccapillaries Poroshell 120 RRHT RRHD agilent com chem sampleprep Agilent...

Страница 80: ...padr o modificada com o objetivo de minimizar o volume morto por isso resultado do teste deve diferir do que pode ser obtido no seu sistema Isso permite avaliar melhor a coluna e garantir uma maior co...

Страница 81: ...Maximum System Pressure Recommended Fitting Part Numbers Up to 400 bar InfinityLab Quick Turn Fitting finger tight Fitting 5067 5966 Up to 800 bar InfinityLab Quick Turn Fitting with Mounting tool Fi...

Страница 82: ...s de seguran a importantes Todos os pontos de conex o em sistemas de cromatografia l quida s o considerados como ponteciais pontos de vazamentos Os usu rios devem estar atentos toxicidade ou inflamabi...

Страница 83: ...s org nicos comuns Recomenda se utilizar uma coluna de guarda para proteger a coluna e aumentar sua vida til As colunas n o devem ser mantidas a um pH elevado ou a altas temperaturas quando n o estive...

Страница 84: ...120 SB C18 StableBond C18 ZORBAX Rx C8 ZORBAX SB Aq ZORBAX SB Phenyl SB C3 Poroshell 300 300SB C3 pH 1 0 a 8 0 90 C StableBond C18 e Poroshell 120 SB C18 80 C Rx C8 e SB Aq SB C3 e SB Phenyl 70 C par...

Страница 85: ...ZORBAX RRHT 1 8 m 600 bar 9000 psi Alta defini o de resolu o r pida ZORBAX RRHD 1 8 m 1200 bar 17 000 psi Todas as colunas ZORBAX Pursuit e Polaris n o indicadas como RRHT ou RRHD e HC C18 2 TC C18 2...

Страница 86: ...sto necess rio um modificador da fase m vel org nica com concentra o mais baixa em compara o com as fases estacion rias tradicionais de cadeias longas do grupo alquila Colunas de mol cula grande ex co...

Страница 87: ...iores que 1 8 m e todas as colunas Pursuit e Polaris inicie o procedimento com um solvente mais forte menos polar 1 Desconecte a coluna do detector e coloque os solventes de lavagem em um b quer 2 Ini...

Страница 88: ...de solvente puro Antes do armazenamento os adaptadores de extremidade devem ser bem fechados com plugues para evitar que o empacotamento seque As colunas podem ser armazenadas de forma segura por per...

Страница 89: ...pidas de alta efici ncia Obtenha mais informa es sobre op es de capilar em agilent com chem lccapillaries Assegure se de que a taxa de coleta de dados esteja otimizada para a sua coluna Utilize uma t...

Страница 90: ...cal information visit agilent com chem columnsupport To place an order visit agilent com chem wheretobuy Agilent offers a complete line of sample preparation products to support LC and LC MS applicati...

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