Agilent Technologies 1260 Infinity, Руководство пользователя

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30 HPLC-Chip Cube Interface User’s Guide
2 Introduction to the Chip Cube
Principle of Operation
Agilent recommends
forward-flush
mode as this mode is less susceptible to
blockages of the enrichment column and additional peak dispersion is
minimal.
In an effort to minimize the hydraulic connections inside the Chip Cube, all
necessary capillaries are supplied with the instrument at appropriate lengths
and diameters. A semi-transparent wide bore tube guides the four capillaries
to the left side of the Chip Cube where they are fed through a recess in the
front cover. A clamp relieves strain on the capillaries.
Opening up the internal flow connections is not required during standard
operation.
Ferrules and Fittings
In the Agilent G4240A Chip Cube, only PEEK micro fittings with 4 mm threads
are used.
However, older Agilent 1260 Infinity Series G2226A nano pumps may require
1/32” double winged fingertight fittings (see:
“Fittings” on page 84).
Table 2 Solvent connection to the Chip Cube
Port No. Capillary Type Remarks
1 n/a currently not used
2 15 µm PEEK coated fused silica
(orange or red) nano pump (analytical pump)
3 75 µm PEEK coated fused silica
(blue) syringe pump
used only with MS calibration & Diagnostic chip
4 n/a currently not used
5 100 µm PEEK coated fused silica
(black)
25 µm PEEK coated fused silica
(yellow) Forward-flush mode
Backflush mode
6 25 µm PEEK coated fused silica
(yellow
100 µm PEEK coated fused silica
(black) Forward-flush mode
Backflush mode