EXC-100 MICROSCOPE SERIES
73 Mall Drive, Commack, NY 11725 • 631
-864-
1000 •
www.accu-scope.com
11
ADJUSTMENT & OPERATION
(continued)
Fig. 11
Adjusting the Condenser (Aperture Diaphragm)
1. The aperture diaphragm determines the Numerical Aperture (N.A.) of
the illumination system. When the N.A. of illumination system matches
the N.A. of the objective, resolution and contrast are optimized. The
aperture diaphragm can also increase the depth of field by closing it
down from the optimal position (resolution is sacrificed by doing this).
2. Turn the condenser lever right or left to change the height of the
condenser and the N.A. of the illumination system (see Fig. 11). The
condenser should be in its highest position during operation.
3. Move the aperture diaphragm lever to the value that is close to the
magnification of the objective (e.g., 10 for the 10x objective). Repeat
for each time a different objective is rotated into the light path.
Fig. 12
Using the 100x Oil Immersion Objective
1. Use the 4X objective to focus the specimen.
2. Place a drop of oil
①
on the specimen observed (see Fig.
12)
3. Rotate the nosepiece counterclockwise and rotate the oil
objective (100X) into the light path. Then use the fine
focusing knob to focus.
★
Make sure there is no air bubble in the oil.
A. Move the eyepiece to examine the air bubble. Open the
aperture diaphragm and field diaphragm fully and observe the
edge of the objective from the tube (It seems round and
bright).
B. Rotate nosepiece slightly and swing the oil objective for
some times to remove the air bubble.
4. After using, wipe the front lens of the objective with a
tissue moistened with a small amount of 3:7 mixture of
alcohol and ether or with dimethylbenzene. Wipe off the oil
on the specimen (cover glass).
★
DO NOT change objectives in the light path before the oil is
wiped off from the slide to avoid getting oil on a non-oil
objective and causing damage to it.
★
Be careful not to use too much solvent to clean the lens as
this may also damage the lens in the long term.