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CELL-DYN
®
3200 System Operator’s Manual
3-15
9140181H—October 2001
Section 3
Principles of Operation
Finally, the instrument evaluates the area below the lymphocyte cluster but above
the hardware threshold (channel 23). Any particles that fall in this area are
separated from the lymphocytes by a dynamic threshold. The following cell types
may be present in this region:
NRBCs
Unlysed RBCs
Giant PLTs
PLT clumps
All particles in this region are excluded from the WBC count and the Differential.
Other Scatterplots
90°/0°
The scatter information is plotted with the 90° scatter on the Y axis and the 0°
scatter on the X axis.
90°D/0°
The scatter information is plotted with the 90°D scatter on the Y axis and the 0°
scatter on the X axis.
90°D/10°
The scatter information is plotted with the 90°D scatter on the Y axis and the 10°
scatter on the X axis.
All scatterplots may be displayed and printed at operator request.
Nuclear Optical Count
Samples containing fragile WBCs are difficult to measure accurately because of
the rapid breakdown of cells during the measurement process. To obtain an
accurate WBC count, an alternate method using the HGB segment (instead of the
WBC segment) is used to measure samples containing fragile WBCs.
The HGB sample segment, after being measured in the HGB Flow Cell, is
transferred to the Optical Flow Cell instead of being sent to a waste chamber as in
normal Patient cycles. While in the HGB Flow Cell, the HGB reagent lyses the
cytoplasmic membrane of the white blood cells but allows the nuclear membrane
to remain intact. This results is a greater stability of the white cells in the sample.
The HGB segment is lysed for approximately 15 seconds before it is sent to the
Optical Flow Cell.
As the HGB segment passes through the Optical Flow Cell, the nuclei of the cells
are counted. The results of this measurement are stored in the Data Log as NOC.
Содержание CELL-DYN 3200
Страница 12: ...xii CELL DYN 3200 System Operator s Manual 9140181K July 2002 NOTES ...
Страница 52: ...1 4 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Use or Function Overview Section 1 NOTES ...
Страница 132: ...3 38 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Principles of Operation References Section 3 NOTES ...
Страница 154: ...5 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Operating Instructions Overview Section 5 NOTES ...
Страница 234: ...5 82 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Operating Instructions Run Menu Section 5 NOTES ...
Страница 300: ...6 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Calibration Procedures Overview Section 6 NOTES ...
Страница 356: ...6 58 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Calibration Procedures References Section 6 NOTES ...
Страница 362: ...8 2 CELL DYN 3200 System Operator s Manual 9140181K July 2002 Hazards Overview Section 8 NOTES ...
Страница 370: ...8 10 CELL DYN 3200 System Operator s Manual 9140181K July 2002 Hazards References Section 8 NOTES ...
Страница 372: ...9 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Service and Maintenance Overview Section 9 NOTES ...
Страница 532: ...11 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Quality Control Overview Section 11 NOTES ...
Страница 558: ...11 28 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Quality Control References Section 11 NOTES ...
Страница 560: ...12 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Printers Overview Section 12 NOTES ...
Страница 566: ...13 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Sample Loader Overview Section 13 NOTES ...
Страница 590: ...14 2 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Reticulocyte Package Overview Section 14 NOTES ...
Страница 662: ...A 4 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Overview Appendix A NOTES ...
Страница 666: ...A 8 CELL DYN 3200 System Operator s Manual 9140181H October 2001 Bar Codes Specifications Appendix A NOTES ...