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CELL-DYN
3000 System Operator’s Manual
3-7
9140240E — May 1995
Chapter 3 Principles of Operation
Figure 3.1: WBC Flow Cell
As the cells enter the flow laser intercept area they scatter the laser light
at different angles, yielding information about cell size, internal
structure, granularity and surface morphology. The optical signals the
cells generate are detected and converted to electrical impulses which are
then stored and analyzed by the computer.
Flow cytometers generally measure two angles of scatter. Forward angle
light scatter is roughly a measure of cell size. Right angle (orthogonal)
light scatter is primarily a measurement of internal granularity.
Combining the information from the two scatter measurements provides
more accurate discrimination between cell populations than either single
measurement. (See
measured by the CELL-DYN 3000.)
Various Angles
of Scattered Light
Sample Stream
Sheath Stream
Focused
Laser Beam
Sample
Feed Nozzle
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Содержание CELL-DYN 3000
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Страница 265: ...6 10 CELL DYN 3000 System Operator s Manual 9140240E May 1995 Calibration Chapter 6 NOTES Search Go Back Book TOC ...
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