4Deep HoloSea S7, Руководство пользователя

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2 INTRODUCTION HoloSea S7
Figure 6: Protective sapphire camera window and copper ring.
Be careful when handling the microscope. At the micron level, smudges on the windows
can greatly affect the quality of the captured holograms. Take care not to smudge the windows.
2.5.2 Chamber 2
This Chamber contains the laser point source, consisting of a 405 nm laser and a fibre optic cable,
which serves as a point source. The point source provides the spherical wave of light necessary
to produce images in inline holography. Similar to the camera in Chamber 1, the point source is
protected by a sapphire glass window (see previous paragraph for care instructions).
2.5.3 Sample Spacer
The Sample Spacer provides a stable environment for imaging microorganisms and particles in
addition to determining the source to screen distance of the instrument, which is essential for
proper data analysis.
The Sample Space, determined by the Sample Spacer, allows for the non-destructive flow of or-
ganisms and particles. The HoloSea’s camera collects holograms of the samples as they, along
with the fluid which contains them, pass through the Sample Space. The standard Sample Space
for a HoloSea is 15 mm.
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