10x Genomics Chromium X Series, Руководство пользователя

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CG000396 | Rev C
Link to TOC | 10xgenomics.com
User Guide | Chromium X Series (X/iX) | Introduction
Introduction
The Chromium X Series is the next generation of instrumentation,
purposefully designed to enable high-throughput experiments.
Compared to the Chromium Controller, the Chromium X Series has:
• Additional sensors, more accurate pressure, and tray temperature control.
• A highly interactive user interface with familiar one-button operation.
• Seamless firmware upgrades, network connectivity and syncing to the
10x Genomics Cloud, to log run information and experimental setups.
• Similar performance as assessed by multiplet rates, library complexity,
read mapping rates, and cell clustering.
• Ability to run an assay with low throughput (LT) or high throughput (HT).
• Low throughput: 100–1,000 cells per channel
• High throughput: 2,000–20,000 cells per channel
2,000–60,000 cells per channel with CellPlex
• Standard
assay: 500-10,000 cells per channel
500-30,000 cells per channel with CellPlex
The Chromium X Series is one instrument, available in two firmware
options (Chromium X and Chromium iX).
Both the standard Next GEM chip and holder (silver color) and the HT Next
GEM chip and holder (black color) are compatible with the instrument.
Comparison of Controller, X, and iX capabilities
Assay Controller iX X
Single Cell 3’ Gene Expression LT
P P P P P P
Single Cell 3’ Gene Expression (Dual Index) P P P P P P
Single Cell 3’ Gene Expression HT No
Upgrade
required
P P
Single Cell Immune Profiling (Dual Index) P P P P P P
Single Cell Immune Profiling HT No
Upgrade
required
P P
Single Cell ATAC
P P P P P P
Single Cell Multiome ATAC + Gene
Expression
P P P P P P
Single Cell 3’ Gene Expression
(Single Index)
P P
Unsupported Unsupported
Single Cell Immune Profiling
(Single Index)
P P Unsupported Unsupported