10x Genomics Chromium X Series, Руководство пользователя

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CG000396 | Rev C
User Guide | Chromium X Series (X/iX) | Tips & Best Practices
Link to TOC | 10xgenomics.com
Icons
Tips & Best Practices section
includes additional guidance
Signifies critical step
requiring accurate execution
HT specific protocol
step updates
For instuctions on the standard assay Next GEM chip, refer to the appropriate assay kit user guide.
Chromium Next GEM Chip Handling
• Minimize exposure of reagents, chips, and gaskets to sources of particles
and fibers, laboratory wipes, frequently opened flip-cap tubes, clothing
that sheds fibers, and dusty surfaces.
• After removing the chip from the sealed bag, use in ≤ 24 h.
• Execute steps without pause or delay, unless indicated. When using multiple
chips, load, run, and collect the content from one chip before loading the next.
• Only even number of reactions can be run on the chip. Refer to the “Load
Chromium Next GEM Chip” section of the appropriate assay kit user
guide for specific instructions.
• Fill all unused paired input wells on a chip with an appropriate volume
of 50% glycerol solution before loading the used wells.
• Avoid contacting the bottom surface of the chip with gloved hands and
other surfaces. Frictional charging can lead to inadequate priming of the
channels, potentially leading to either clogs or wetting failures.
• Minimize the distance that a loaded chip is moved to reach the
Chromium instrument.
• Keep the chip horizontal to prevent wetting the gasket with oil, which
depletes the input volume and may adversely affect the quality of the
resulting emulsion.
Chromium X Chip Holders
• Chromium X Chip Holders encase Chromium Next GEM Chips used for
the HT (high throughput assay).
• The holder lid flips over to become a stand, holding the chip at 45
degrees for optimal recovery well content removal.
• Squeeze the sliders on the back side of the holder together to unlock the
lid and return the holder to a flat position.
Chromium
Next GEM Chip
Troubleshooting section
includes additional guidance