ZYMO RESEARCH CORP.
Phone: (949) 679-
1190 ▪ Toll Free: (888) 882-9682 ▪ Fax: (949) 266-9452 ▪ [email protected] ▪ www.zymoresearch.com
Page 1
Product Contents:
ZymoPURE
™
Plasmid Miniprep
Kit Size
D4209
50 preps.
D4210
100 preps.
D4211
400 preps.
D4212
800 preps.
Storage
Temperature
ZymoPURE
™
P1
1
(Red)
13 ml
2x 13 ml
100 ml
210 ml
4°C
ZymoPURE
™
P2
2,3
(Green)
13 ml
2x 13 ml
100 ml
210 ml
Room Temp.
ZymoPURE
™
P3 (Yellow)
13 ml
2x 13 ml
100 ml
210 ml
Room Temp.
ZymoPURE
™
Binding Buffer
3
14 ml
2x 14 ml
110 ml
2x 110 ml
Room Temp.
ZymoPURE
™
Wash 1
2x 20 ml
4x 20 ml
320 ml
2x 320 ml
Room Temp.
ZymoPURE
™
Wash 2
4
12 ml
23 ml
3x 28 ml
6x 28 ml
Room Temp.
ZymoPURE
™
Elution Buffer
2x 1 ml
6 ml
12 ml
30 ml
Room Temp.
Zymo-Spin
™
II-P Columns
50
100
400
800
Room Temp.
Collection Tubes
50
100
400
800
Room Temp.
Instruction Manual
1
1
1
1
-
Note -
Integrity of kit components is guaranteed for up to one year from date of purchase. Reagents are routinely
tested on a lot-to-lot basis to ensure they provide maximal performance and reliability.
1
ZymoPURE
™
P1 contains RNase A (100 µg/ml) and is stable at room temperature without loss in RNase activity,
however, for long-term storage the product should be stored at 4-8° C.
2
Caution: ZymoPURE
™
P2 Buffer contains NaOH. Please use proper safety precautions.
3
The ZymoPURE
™
P2 and ZymoPURE
™
Binding Buffer may have precipitated. If this occurs, dissolve the precipitate
by incubating the bottles at 30-37 ºC for 10-20 minutes and mix by inversion. Do not microwave!
4
ZymoPURE
™
Wash 2 included with D4208S is supplied ready-to-use and does not require the addition of ethanol
prior to use. ZymoPURE
™
Wash 2 included with D4209, D4210, D4211, and D4212 are supplied as a concentrate and
require the addition of ethanol prior to use. See Buffer Preparation (page 4) for instructions.
Specifications:
•
DNA Purity:
Eluted DNA is ultrapure, endotoxin-free, and well suited for transfection,
transformation, sequencing, restriction endonuclease digestion,
in vitro
transcription, and
other sensitive applications.
o
Typical Abs
260/280
≥ 1.8 and Abs
260/230
≥ 2.0
•
Plasmid DNA Yield:
Up to 100 µg per preparation
(Actual yield is dependent on the plasmid
copy number, culture growth conditions, and strain of E. coli utilized)
•
Plasmid DNA Size:
Up to 25 kb
•
Recovery Volume:
≥ 25 µl of ZymoPURE
™
Elution Buffer or DNase free water
•
Required Equipment:
Microcentrifuge and/or vacuum manifold (recommended).
•
Processing Time:
15 min
Satisfaction of all Zymo
Research products is
guaranteed. If you are
dissatisfied with this product,
please call 1-888-882-9682.
Notes:
This product is for research use
only and should only be used by
trained professionals. It is not for
use in diagnostic procedures.
Some reagents included with this
kit are irritants. Wear protective
gloves and eye protection.
Follow the safety guidelines and
rules enacted by your research
institution or facility.
™ Trademarks of Zymo Research
Corporation.
Several ZymoPURE
™
product
technologies are subject to U.S.
and foreign patents or are patent
pending.
pGL3
™
is a registered trademark
of Promega Corporation.