www.zinexts.com 30 MagPurix 12 EVO Instruction Manual
pipetting failure
material or excess DNA
in sample causing
clumps or aggregates
recommended amount of starting material as
listed in the Reagent Kit manual (Handbook).
2.
Suggest using blood kit 1200 instead of blood kit
200 (if testing sample is blood)
DNA Quality Problems
Problem
Cause
Solution
Low DNA yield
Incomplete lysis
Decrease the amount of starting material used.
Be sure to add Proteinase K during lysis, if included in the
protocol.
Make sure that the sample is completely immersed in the
Lysis Buffer.
Poor quality of starting
material
Be sure to process the sample immediately after collection
or store the sample at the appropriate temperature. The
yield and the quality of DNA isolated depend on the starting
material.
Insufficient amount of
magnetic beads added
During shipping, some magnetic bead solution may adhere
to the sealing foil of the cartridge. To collect any bead
solution from the foil, tap the cartridge to deposit the bead
solution at the bottom of the well.
Clogged Tips resulting
in DNA loss
Ensure that the lysate does not contain any particulate
material that can clog the tip sprout. If needed, centrifuge
the sample prior to the MagPurix purification.
No DNA
recovered
Magnetic beads stored
or handled improperly
Store cartridge containing the beads at room temperature.
Do not freeze the cartridge as the beads may be irreversibly
damaged.
Make sure that the beads are in solution at all times and do
not dry. Dried beads are non-functional.
Eluate containing
DNA
is discolored
Magnetic beads present
in the eluate
Remove any magnetic beads using a magnetic separator or
centrifuge the sample in a microcentrifuge for 1 minute at
maximum speed.
DNA contaminated with
heme
Minimize the amount of blood or blood-stained sample
used (≤ 20μl blood spot for forensics sample).
DNA is sheared
or degraded
Bubbles form during
mixing steps
To prevent bubble formation during mixing, make sure the
sample volume is at least the recommended volume listed
