Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
ZEISS Center Screen Area / Image Containers - Display and Image Analysis LSM 880
482 000000-2071-464 10/2014 V_01
6.14 Mean of ROI: Additional View Type for Time Series
The Mean of ROI View Type allows to
−
display the intensity - time diagram (mean intensity in user defined ROIs over time),
−
use frame - time series and frame - Z-Stack - time series as input,
−
show the intensity values in table form and copy table to clipboard or save as text file.
The Mean of ROI function permits interactive
analysis and monitoring intensity over time.
The settings of the
Dimensions, Display , Player
and
Graphics
View Options control blocks apply.
The additional view-specific Mean of ROI View
Option
control block is shown in Fig. 675.
Any changes done with this View Option control
block are effective immediately.
The Image Display in the Mean of ROI View shows
3 panels: the intensity-over-time diagram, the data
table with the intensity values for each ROI over
time and the image display with ROIs in overlay
(see Fig. 676 and Fig. 677).
To access the Mean of ROI View Type, load or acquire a time series data set and click on the Mean ROI
View tab.
The mean intensity of one or several regions of interest is displayed over time. Time flags from bleaching
experiments of trigger events are marked with little symbols on the time-axis of the graph (see Fig. 676).
The
Mean of ROI analysis always refers to the currently displayed z-section of a Z-Stack (see Dimension
View Option control block for which section it is).
Fig. 675 Mean of ROI View Option control
block