Zeiss LSM 880, Operating Manual

Page: 423 / 690

CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
10/2014 V_01 000000-2071-464 417
5.5.6.2 FCS Options – Measure
Move Stage During Measure
If the
Move stage during measure
check box is
activated, the stage will move during a measure-
ment (Fig. 587).
This option allows you to move the stage forth and
back during the entire measurement time. This
option is useful during rare event detection when
large, slowly moving objects in extremely small
concentrations have to be registered. Since these
aggregates are diffusing very slowly without such
movement the measurement times would be
prohibitively long. It should be taken into account
that, in this case, the data stream is analyzed with
the coincidence analysis rather than by correlation
analysis.
If the
Move stage during measure check box is ticked, the following settings are possible in the dialog:
−
Travel distance of the stage in millimeters: Only integer numbers of millimeters are possible. Type
into the
Distance x
and
Distance y
display boxes or use the arrowheads.
−
Speed of the stage: Only the three preset stage speeds 0.48 mm/s, 4.81 mm/s and 24.01 mm/s are
possible. Select 1, 2 or 3 from the
Speed
dialog box using the pull down menu.
−
Travel direction: The stage can be moved along the x- or y-axis of the stage. In this case the value in
Distance x or Distance y are set to 0, respectively.
Turning points in the stage movement and turbulent medium can cause correlation signals. Watch
out for these artifacts.
Control AOTF Dampening Automatically
If the
Control AOTF dampening automatically
check box is ticked, the laser AOTF setting will be
extended into the lower dynamic range. Please note that in case two activated laser are set at different
ranges, the one in the lower range will be set to the lowest available AOTF of the higher range.
If the box is unchecked, the AOTF can be suppressed overall by a certain percentage. By moving the slider
of the
AOTF Power (%) selection box, the respective dampening factor can be set.
Fig. 587 FCS Options, Measure