Zeiss LSM 880, Operating Manual

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CHAPTER 1 - SYSTEM OPERATION
LSM 880 Left Tool Area and Hardware Control Tools ZEISS
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Weighted unmixing:
When this option is checked, spectral channels with high noise contribute less to the unmixing result. This
option includes a statistical analysis of the signal-related (Poisson-) noise and weighs the respective
contribution for the fitting with the combination of reference spectra to the experimental data. Note: this
option involves a more sophisticated unmixing algorithm and therefore takes longer than the basic
unmixing analysis. Weighted unmixing generates improved unmixing results when acquisition channels
are not so well balanced but still have a good signal-to-noise ratio.
Display channels with statistical confidence:
This option displays an additional channel per unmixed component which shows the relative statistical
error in each unmixed component channel. This statistical uncertainty of the pixel intensity in the
unmixed channel is calculated based on the (Poisson-) noise from the acquired (input-) channels, the
bandwidth and –position and the quality of the reference spectra. The display can be interpreted best
when an appropriate false color LUT is chosen. The error is displayed as percentage of unmixed channel
pixel intensity. For example: A "confidence value", i.e., pixel intensity of 50 represents a 50% statistical
uncertainty of the corresponding intensity in the unmixed channel. The higher the statistical error for the
acquired pixel intensity (the shot noise), the higher is the intensity value of this pixel.
Fig. 412 Image Display window before unmixing in Preview
Fig. 413 Image Display window after unmixing