ELYRA 7
Level-adjustable Sample Holder with Insert for Piezo Stage
ZEISS
03/2019 V_02
000000-2262-999
17
3.
Adjust leveling in X in the following way:
a)
Enter in the
Stage
tool in the
Step [
µ
m]
input box "1000" (Fig. 10). Press the
upper or lower pointing arrow of the
X
input box, whatever is required to step
1000 µm to the right X direction.
b)
If the sample is out of focus (Fig. 7/
B
)
refocus manually by using the stand’s
focus drive (Fig. 7/
C
); otherwise no further
leveling is necessary. Record the relative
change in the focus position by reading
the value in the
Z-Position
input box of
the
Focus
tool. If the focus is off more
than 10 µm it is recommended to do a
pre-adjustment. To this end adjust the
edges of the clefts viewed from the right
side (Fig. 6/
csv
) and back side (Fig. 6/
cbv
)
to be parallel using screws
sy
and
sx
,
respectively (Fig. 6). The cleft’s width
should be approximately 2 mm as preset
by the factory, but can be adjusted with
screw
sz
if needed.
c)
Multiply that value with
55
and manually
set the focus onto this value using the
stand’s focus drive (Fig. 7/
D
). (Note: The
factor
55
is only accurate, if the sample
was positioned in the center.)
d)
Use the back left screw (Fig. 6/
sx
) of the
sample holder to readjust the focus
(Fig. 7/
E
). Turn clockwise if the focus
position was positive, counter clockwise if
it was negative. (Note that one turn
corresponds to approximately 125 µm).
e)
Press the lower pointing arrow of the
X
input box of the
Stage
tool to return to
the initial position. If the image is in focus
(Fig. 7/
F
) X leveling is complete; otherwise
repeat steps 3a) - e) iteratively. If
applicable, the multiplication factor of 3c)
can be adjusted.
4.
Adjust leveling in Y in the following way:
a)
Enter in the
Stage
tool in the
Step [
µ
m]
input box "1000". Press the upper or lower pointing
arrow of the
Y
input box, whatever is required to step 1000 µm upwards in the Y direction
A
).
b)
If the sample is out of focus (Fig. 7/
B
) refocus manually by using the stand’s focus drive
C
); otherwise no further leveling is necessary. Record the relative change in the focus
position by reading the value in the
Z-Position
input box of the Focus tool.
c)
Multiply that value with
40
and manually set the focus onto this value using the stand’s focus
drive (Fig. 7/
D
). (Note: The factor
40
is only accurate, if the sample was positioned in the center.)
Fig. 9
Focus tool – z-position set to zero
Fig. 10
Stage tool – step size X set to 1000