ELYRA 7
Left Tool Area and Hardware Control Tools
ZEISS
03/2019 V_02
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After the end of the Experiment
Periodic
stabilization remains switched off.
An active
Find Surface
becomes deactivated by pressing both,
Continuous
or
Start Experiment
and
remains inactive after the scan finished.
Only if the
Start Experiment
button has been pressed synchronous stabilization will be effective.
5.2.13
Focus Devices and Strategies for
Widefield Imaging Modes
You can opt for (Fig. 171):
−
Off
, no holding focus
−
Definite Focus
, hardware holding focus
If
Definite Focus
is selected, the
Autofocus
every n Timepoint
check box becomes available.
Check the box to operate the Definite Focus in
synchronous mode (Fig. 172).
Define the cycles in number of frames in which the
Definite Focus will be activated in the
Autofocus
every n Timepoint
input box.
We strongly recommend to use the Definite Focus in asynchronous and continuous mode operated
by the TFT display for stabilization in SMLM experiments to minimize drift as best as possible. Note,
in synchronous mode started from ZEN Software, the actual z-position at the time the Definite
Focus will start is kept as the reference point. Hence, changing the focus will result in a
compensation by the Definite Focus the next time it is active. In contrast, in asynchronous mode
started from the TFT display, changing the focus by the stands focus drive will set this focus as the
new reference point.
Fig. 171
Focus strategies for Laser WF imaging
mode
Fig. 172
Definite Focus menu