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Using the PeCon CO2 system
The full manual is located on our core web pages and as a printed copy with
the spinning disk system manuals. The CO2 controller should be switched on
15 minutes before use
and allowed to stabilise before the CO2 feed is
activated.
Operation
•
Switch on the CO2-Controller. Power on/off on the back of the device.
The CO2-Controller should have a lead time of approx. 15 minutes
before the CO2 is fed in.
•
After approx. 5 min. the CO2 value in the display shows 0.0. This zero
value can be corrected with the potentiometer "sensor calibration" on
the back of the device (see section maintenance).
•
Press the "display" button to view the CO2 nominal value. The LED
should mark "nom". Press the “+" and "–" buttons to set the nominal
value. To view the CO2 real value press the button "display" again.
•
Regulate the CO2 influx with the CO2 reducing valve. The valve should
be adjusted carefully to avoid a permanent overshoot of the CO2 value
(see guideline table 1).
•
The carrying capacity of the pump can be chosen by pressing the "pump"
button. The power is increased or decreased by repeated pushing. For
5% CO2, a pump power of 2 is recommended.
•
Activate the CO2 control by pressing the "valve" button. This button has
a two-
color LED, Red → no gas influx and Green → CO2 flow into the
mixing chamber.
•
Press the button "valve" again to deactivate the CO2 control. The LED in
the button is off.
The PeCon CO2 controller is
on loan
from the Zeiss 510 confocal system and
may have be used on other microscope systems occasionally (principally with
the Leica SP8 confocal).
