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Using the Zeiss Observer XL stage incubator
The Zeiss 37oC incubator controller
must be switched on before any other
equipment
otherwise the control link to the microscope stand won’t be
established and ‘Incubation’ will not appear as a tab in the Observer Z1’s LCD
controller.
To switch on the Temp Controller press its rear rocker switch
to the left
(the
lowest LED lights up green). Then switch on the upper Heating Unit XLS by
pressing its rear rocker switch
to the right
- the middle yellow ‘heating on’ LED
will light up. To set the microscope stage incubator temperature press Home,
Microscope, and Incubation on the microscope’s LCD panel.
If you are using the heated incubator at 37oC you should switch on the
incubator at least 2 hours before imaging. For the most stable time-lapse
imaging at 37oC leave the incubator on overnight.
The spinning disk system has the Zeiss ‘Definite Focus’ NIR LED hardware
autofocus system that fires an LED at the coverslip and keeps that distance
constant. This only works well if your cells are growing are on top of the
coverslip.
