Zeiss ApoTome.2 User Manual Download

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Summary of Contents for ApoTome.2

Page 1: ...Operating Manual ApoTome 2...

Page 2: ...ate service Unless expressly authorized forwarding and duplication of this document and the utilization and communication of its contents are not permitted Violations will entail an obligation to pay...

Page 3: ...g principle 16 2 6 Suitability of objectives for the use of ApoTome 2 17 2 7 Technical data 21 3 STARTUP 23 3 1 Microscope stand requirements 23 3 2 Unpacking ApoTome 2 23 3 3 Connecting the control b...

Page 4: ...Carl Zeiss Contents ApoTome 2 4 423667 7144 001 05 2012 6 APPENDIX 31 6 1 List of abbreviations 31 6 2 Index 32...

Page 5: ...2 System overview ApoTome 2 for Axio Observer and Axiovert 200 13 Fig 2 3 System overview ApoTome 2 for Axio Zoom V16 14 Fig 2 4 Components of ApoTome 2 15 Fig 2 5 Changer with transmission grids 16 F...

Page 6: ...iss specifications plus monitor operating system Windows XP Windows Vista 32 bit version or Windows 7 32 bit version ApoTome 2 slider with ApoTome control box Imaging software AxioVision 4 8 2 control...

Page 7: ...a hazard to the user of the instrument CAUTION Hot surface CAUTION Emitted LED radiation CAUTION Disconnect the instrument from line power before opening it CAUTION This symbol is a warning that indi...

Page 8: ...ioactive specimens or other specimens that could be hazardous to health When handling such specimens observe all legal regulations particularly the relevant national regulations for the prevention of...

Page 9: ...se fire Always keep the ventilation slots clear and make sure that no objects enter the instrument through these slots The instruments may only be operated by trained personnel who must be aware of th...

Page 10: ...antee is provided for defects caused by natural wear wearing parts in particular and improper use The instrument manufacturer is not liable for damage caused by faulty operation negligence or any othe...

Page 11: ...it can be connected directly to the microscope by a CAN BUS cable In this case the ApoTome 2 directly communicates with the PC via the electronic system of the microscope If an Axiovert 200 is used t...

Page 12: ...INSTRUMENT DESCRIPTION Carl Zeiss System overview ApoTome 2 12 423667 7144 001 05 2012 2 3 System overview 2 3 1 ApoTome 2 for Axio Imager Fig 2 1 System overview ApoTome 2 for Axio Imager...

Page 13: ...TRUMENT DESCRIPTION ApoTome 2 System overview Carl Zeiss 05 2012 423667 7144 001 13 2 3 2 ApoTome 2 for Axio Observer and Axiovert 200 Fig 2 2 System overview ApoTome 2 for Axio Observer and Axiovert...

Page 14: ...INSTRUMENT DESCRIPTION Carl Zeiss System overview ApoTome 2 14 423667 7144 001 05 2012 2 3 3 ApoTome 2 for Axio Zoom V16 Fig 2 3 System overview ApoTome 2 for Axio Zoom V16...

Page 15: ...me 2 Carl Zeiss 05 2012 423667 7144 001 15 2 4 General view of ApoTome 2 1 Slider ApoTome 2 for Axio Imager 2 Slider ApoTome 2 for Axio Observer and Axiovert 200 3 Slider ApoTome 2 for Axio Zoom V16 4...

Page 16: ...fferent intensity depending on the phase position of the transmission grid The three images captured see Fig 2 6 with the grid structures are calculated so that an optical section with increased contr...

Page 17: ...2 2 1 4 1 2 0 8 0 7 EC Plan Neofluar 63x 0 95 Air 1 0 1 1 0 6 0 7 0 4 0 4 EC Plan Neofluar 63x 1 25 Oil 1 6 1 5 0 9 0 9 0 5 0 5 EC Plan Neofluar 100x 1 3 Oil 1 0 0 9 0 6 0 5 0 4 0 3 LCI Plan Neofluar...

Page 18: ...6 5 1 7 3 8 0 9 2 0 LCI Plan Neofluar 63x 1 3 Water or glycerin 1 5 1 3 0 9 0 8 0 5 0 4 Plan Apochromat 10x 0 45 Air 4 2 20 2 2 4 11 7 1 3 6 1 Plan Apochromat 20x 0 8 Air 3 1 4 8 1 8 2 8 1 0 1 5 Plan...

Page 19: ...1 5 5 3 1 0 3 6 0 8 2 8 Plan Neofluar 2 3x 250 1 1 3 2 0 7 2 2 0 6 1 8 1 In ZEN blue edition no preset zoom positions The following filter sets are recommended if ApoTome 2 shall be used with Axio Zo...

Page 20: ...eGFP eYFP 489047 9901 000 FS 47 HE BP 436 25 FT 455 HE BP 480 40 CFP 488049 9901 000 FS 49 G 365 FT 395 BP 445 50 DAPI 488050 9901 000 FS 50 BP 640 30 FT 660 BP 690 50 Alexa 647 Alexa 660 Cy5 489059...

Page 21: ...40 to 70 C Storage Permissible ambient temperature 5 to 40 C Permissible relative humidity without condensation max 75 at 35 C Operation Permissible ambient temperature 5 to 40 C Permissible relative...

Page 22: ...012 Fuses as per IEC 127 ApoTome 2 control box T 2 A H 250 V 5x20 mm Grid frequencies Axio Imager slider transmission grids 1 2 3 5 9 17 5 lp mm Axio Observer Axiovert 200 slider transmission grids 1...

Page 23: ...icroscope should be installed as free from vibrations as possible on an anti vibration table or a suitable microscope support 3 2 Unpacking ApoTome 2 The ApoTome 2 slider is delivered in a polyethylen...

Page 24: ...n two different ways Via USB cable directly at the PC Use a USB cable to connect the USB port 3 1 1 on the rear panel of the control box with a free USB port at the PC used Via CAN BUS cable to a free...

Page 25: ...poTome 2 Carl Zeiss 05 2012 423667 7144 001 25 4 OPERATION 4 1 Control and functional elements of ApoTome 2 1 Click stop position 1 free passage 2 Click stop position 2 transmission grid 3 On off swit...

Page 26: ...ing the slider Carefully insert the ApoTome 2 slider in the plane of the luminous field diaphragm of the reflected light beam path of the microscope used On insertion a short beep signal is generated...

Page 27: ...onger oily or fatty contamination immersion oils fingerprints with a cotton bud lens cleaning paper or a dust free cotton cloth using optics cleaning agent L This cleaning agent is made of 90 percent...

Page 28: ...nt use Have damages repaired by appropriately qualified staff 5 2 2 Replacing fuses Before replacing fuses make sure to disconnect the power cable The fuse compartment is located at the rear panel of...

Page 29: ...e position click position 2 transmission grid not focused To obtain an artifact free result image the transmission grid must be imaged with a high quality in the specimen plane For this purpose calibr...

Page 30: ...med by Carl Zeiss Service staff or specially authorized personnel To ensure optimum setting and trouble free function of your microscope over a longer period of time we recommend that you enter into a...

Page 31: ...Association DIN Deutsches Institut f r Normung German Institute for Standardization EC European Community EN European standard HBO Mercury vapor short arc lamp for fluorescence IEC International Elect...

Page 32: ...g the control box 24 Control and functional elements 25 D Designation 11 Device safety 7 Dimensions 21 G General view 15 I Inserting the slider 26 Instrument description 11 Intended use 11 L Line volt...

Page 33: ...2 423667 7144 001 33 S Startup 23 Storage 21 Switching off 26 Switching on 26 System overview 12 13 14 T Technical data 21 Tools 29 Transmission grid 16 Troubleshooting 29 U Unpacking 23 W Warning lab...

Page 34: ......

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