YMC Europe GmbH
Operation
YMC Pilot
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Low pressure chromatography column
The column bed is visually compressed.
23. Check and mark the bed length frequently.
24. If the final bed length is reached, stop the pump.
25. Turn the piston of the adjustable end piece in down direction until the column
bed is reached.
26. Start the pump.
27. Check, if the column bed compresses further. A dead volume would occur.
28. If a dead volume occurs, stop the pump and repeat steps 22 to 25.
29. If no dead volume occurs, the glass column is released Chapter 6.5, p. 32.
30. Equilibrate the glass column with the desired eluent and test the column
performance.
6.4.3
Slurry column packing with sedimentation
The column is packed via the sedimentation slurry method as follows:
1. Remove the adjustable end piece Chapter 6.3.1, p. 27.
2. Tighten the O-ring of the fixed end piece Chapter 6.1, p. 26.
3. Connect the pump / system with the tubing to the fixed end piece.
4. Start the pump.
5. Fill the glass body with solvent or buffer until 2-3 cm above the frit in the fixed
end piece (bottom-up).
This ensures that no air is trapped under or inside the frit.
6. Close the valve in the tubing of the fixed end piece.
7. Pour the homogenous and / or degassed slurry into the glass body.
8. Rinse the inner wall of the column body with slurry solvent for removal of
particles.
By removing particles from the inner wall of the column body, a correct seal
with the O-ring is guaranteed.
9. Do not move the glass column until packing is completed.
YMC recommends covering the open column body during sedimentation
with some plastic film.
10. Wait until the packing material has settled (sedimentation).
Depending on the packing material, this can take several hours.
11. Fill the glass column up to the edge of the column body with slurry solvent.
12. Connect the pump to the adjustable end piece.
13. Flush the piston of the adjustable end piece with slurry solvent until the frit in
the adjustable end piece is wetted completely.
14. Stop the pump.
15. Insert the adjustable end piece with loosened O-ring into the column.
By inserting the adjustable end piece with loosened O-ring into the solvent,
the risk of air bubbles below the frit is reduced.
