YMC Europe GmbH
Operation
YMC Pilot
30 / 49
Low pressure chromatography column
6.4.2
Slurry column packing under flow
The column is packed with the slurry method under flow as follows:
1. Remove the adjustable end piece Chapter 6.3.1, p. 27.
2. Tighten the O-ring of the fixed end piece Chapter 6.1, p. 26.
3. Connect the pump / system with the tubing to the fixed end piece.
4. Start the pump.
5. Fill the glass body with solvent or buffer until 2-3 cm above the frit of the fixed
end piece (bottom-up).
This ensures that no air is trapped under or inside the frit.
6. Close the valve on the tubing of the fixed end piece.
7. Connect the pump to the adjustable end piece.
8. Flush the piston of the adjustable end piece with slurry solvent until the frit in
the adjustable end piece is wetted completely.
9. Disconnect the pump from the adjustable end piece.
10. Pour the homogenous and / or degassed slurry into the glass body.
11. Rinse the inner wall of the column body with slurry solvent to remove particles
from the walls.
By removing particles from the inner wall of the column body, a correct seal
with the O-ring is guaranteed.
12. Fill the glass column up to the edge of the column body with slurry solvent.
13. Loosen the O-ring of the adjustable end piece.
14. Insert the adjustable end piece with loosened O-ring into the column.
By inserting the adjustable end piece with loosened O-ring into the solvent,
the risk of air bubbles below the frit is reduced.
Check that no air bubbles are below the frit.
15. Turn the cylinder screws with the hex-wrench through the closing disc into the
column disc. This way, the column is closed.
16. Tighten the O-ring of the adjustable end piece.
17. Open the valve in the tubing on the adjustable end piece.
18. Turn the piston of the adjustable end piece in the down direction until the
tubing is completely filled with liquid.
The tubing is now free of air.
19. Connect the pump with the tubing to the adjustable end piece.
20. Open the valve in the tubing of the fixed end piece.
21. Start the flow (top-down).
22. Increase the flow rate stepwise until the desired pressure / flow rate is
reached. Wait after every step until the pressure is constant. Afterwards, the
flow rate can be further increased.
Consider the manufacturer´s recommendations for the packing material.
The flow rate should be considerably (>20%) higher than the required
operating flow rate.
