WPI MICRO-ePUMP, Instruction Manual

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MICRO-ePUMP
World Precision Instruments 11
Fig. 15—The Microelectrode Interface Cable has two connectors, one for the reference
electrode and one for the microelectrode holder.
• The red pin connects to the 2 mm socket on the microelectrode holder.
Insert the red pin into the socket on the microelectrode holder.
Fig. 16—The red pin of the microelectrode interface cable connects to the microelectrode
holder.
• The black pin of the microelectrode interface cable connects to the 2 mm
socket on one of the reference wires. This is necessary to complete the
electrical connection to solution. For the well-style electrode, insert the black
pin into the socket of the reference electrode (Fig. 17). For the probe-style
electrode, the black pin of the microelectrode interface cable connects to
the back side of the reference electrode block. The probe-style electrode pin
plugs into the front of the block (Fig. 18).
Fig. 17—(Left) The well-style electrode is properly connected. [Ring reference electrode – OD:
12.7 mm (0.5”), Thickness: 0.4 mm (0.16”)]
Fig. 18—(Right) The probe-style electrode is properly connected.
3. A. Well-style electrode –If you use the well-style electrode, the gold ring must be
secured to your slide using vacuum grease to create an impermeable temporary
seal. Place the liquid medium with your embryos inside the gold ring.
B. Probe-style electrode –If you are using the probe-style electrode, place the
block near the injection site. Then, bend the bare reference wire as needed to
make contact with the liquid medium in order to complete the circuit.