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WORLD

PRECISION

INSTRUMENTS

MICRO-ePUMP

Microinjector

Serial No._____________________

www.wpiinc.com

INSTRUCTION MANUAL

012220

Summary of Contents for MICRO-ePUMP

Page 1: ...WORLD PRECISION INSTRUMENTS MICRO ePUMP Microinjector Serial No _____________________ www wpiinc com INSTRUCTION MANUAL 012220...

Page 2: ......

Page 3: ...mpensation Pressure 16 Manufacturing Micropipettes 18 Calibrating Volume by Measuring Droplet 18 Calibrating Rate using a Known Volume 19 Multibarrel Microinjection 20 MAINTENANCE 21 Cleaning 21 ACCES...

Page 4: ...ii World Precision Instruments...

Page 5: ...ION Designed to simplify intracellular injection and a variety of other micro in jec tion tasks the MICRO ePUMP uses carefully regulated air pres sure for injecting cells with fluid Injected volumes r...

Page 6: ...nce being injected A reference electrode is used to place the media at 0 0 V potential with reference to the generated voltage The MICRO ePUMP offers separate regulated compensation back filling preve...

Page 7: ...r Adapter 1 Instruction Manual available online at www wpiinc com manuals Unpacking Upon receipt of this instrument make a thorough inspection of the contents and check for possible damage Missing car...

Page 8: ...pette tip a parameter to modify it using the knob or the settings area to adjust the settings Adjustment Knob Rotate the adjustment knob to change the highlighted parameter Press it to toggle between...

Page 9: ...switch icon Press the foot switch to initiate a timed sequence timed mode or to manually eject pressure manual mode This foot switch is a two stage switch Depress it half way down to engage the MICRO...

Page 10: ...used protocols in the MICRO ePUMP memory If the box is empty no protocol is loaded Injection Counter Every time you depress the foot switch all the way when the port is enabled the injection counter...

Page 11: ...ration with minimal trauma The researcher determines the amplitude and frequency of the signal that best suits the application If you are using the MICRO ePORE techcnology touch the parameter and rota...

Page 12: ...eters as desired Then press one of the Save buttons For example press SAVE1 to save the parameters as Protocol 1 To load a set of saved parameters a protocol press the appropriate LOAD button The numb...

Page 13: ...ng connected to the barb Body Fig 11 The 300744 Capillary Kit must be properly assembled 1 Slide the bare end of the Adapter Tubing Assembly through the Pipette Handle 2 Connect it to the barb of the...

Page 14: ...with a slight clockwise twist Fig 12 Left Align the connector with the Injection Port slide the connector in position and rotate the connector a little clockwise to lock it into place Fig 13 Right Th...

Page 15: ...electrode Fig 17 For the probe style electrode the black pin of the microelectrode interface cable connects to the back side of the reference electrode block The probe style electrode pin plugs into...

Page 16: ...ameter by turning the knob To change the dial adjustment speed press the knob on the front of the MICRO ePUMP The snail changes the display in 0 01 increments and the running man changes it in increme...

Page 17: ...this configuration No parameters show in the bottom half of the screen 2 Tap a parameter and turn the knob to change the parameter Fig 24 Touch the Frequency parameter or the Amplitude parameter and...

Page 18: ...shows the injection count Whenever the foot switch is fully depressed the injection counter increments Reset it to zero by pressing on the Injections area of the main display touchscreen Adjust the M...

Page 19: ...from the main screen press the SAVE1 SAVE2 or SAVE3 button The parameters are saved in Protocol 1 Protocol 2 or Protocol 3 depending on which SAVE button you chose 4 Press the BACK button to return to...

Page 20: ...Capillary Kit on page 9 5 When the micropipette is properly installed click on the Settings area to access the Settings menu and press the Port OFF button to enable the Injection Port TECHNIQUES IN MI...

Page 21: ...the contact angle for glass and water is zero unless the glass surface is treated From this equation we see that the smaller the inside diameter the greater the capillary action The capillary pressure...

Page 22: ...on becomes prohibitively large and the hydrophilic surface of the glass greatly limits the flow of fluid through the tip Silanization decreases the surface tension and allows the fluid to flow smoothl...

Page 23: ...te of injection For example if the volume in 1 mm of glass with 0 58 m ID WPI 1B100 is 264 nL and it takes 30 seconds to inject that volume then the flow for 1 second is 264 nL 30 s or 8 8 nL second a...

Page 24: ...vital to making a correct injection of 1 nL 11 Divide the average found in step 9 by the calculated volume in nL of a 1 mm length of your pipette found in step 10 This establishes the time in millise...

Page 25: ...nd other contamination There is no need to clean the inside Use a soft cloth dampened with a mild solution of detergent and water Do not use abrasive cleaners ACCESSORIES Part Number Description 75122...

Page 26: ...n Port connector call WPI technical support 104 Tank pressure is too low to deliver the desired injection pressure Tank depleted due to previous injections or leak Wait for pump to charge Check all pr...

Page 27: ...ping Weight 90 264 V 50 60 Hz 48 W 213 w x 207 D x 250 H mm 8 4 x 8 1 x 9 8 5 kg 11 lb Ambience Ambient Temperature Relative Humidity For indoor use only 15 C 40 C 10 75 non condensing Both Backfillin...

Page 28: ...1 mm 1 24 mm 100 nL 464 m 575 36 m 10 nL 215 m 267 m 1 nL 100 m 1 24 m 100 pL 46 m 57 m 10 pL 21 5 m 27 m 1 pL 10 m 12 4 m Fig 37 This graph compares the dimensions of a cube and a sphere with the sam...

Page 29: ...ing of ion sensitive microelectrodes J Neuroscience Methods 8 231 247 1983 Deyhimi F and J A Coles Rapid silylation of a glass surface Choice of reagent and effect of experimental parameters on hydrop...

Page 30: ...26 World Precision Instruments DECLARATION OF CONFORMITY...

Page 31: ...special or consequential arising from a failure of this product to operate in the manner desired by the user WPI shall not be liable for any damage to data or property that may be caused directly or...

Page 32: ...n Germany Tel 49 0 6031 67708 0 Fax 49 0 6031 67708 80 E mail wpide wpi europe com China Hong Kong WPI Shanghai Trading Co Ltd Rm 27A Liangfeng Building No 8 Dongfang Road Pudong Free Trade Zone Shang...

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